Study of CD27, CD38, HLA-DR and Ki-67 immune profiles for the characterization of active tuberculosis, latent infection and end of treatment
Díaz-Fernández, Sergio (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Villar-Hernández, Raquel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Stojanovic, Zoran (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Fernández, Marco A. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
De Souza Galvão, Maria Luiza (Hospital Universitari Vall d'Hebron)
Tolosa, Guillermo (Universidad de La Frontera (Xile))
Sánchez-Montalvá, Adrián (Hospital Universitari Vall d'Hebron)
Abad Capa, Jorge (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Jiménez-Fuentes, María Ángeles (Hospital Universitari Vall d'Hebron)
Safont Gonzalez, Guillem (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Romero i Andrada, Iris (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Sabrià, Josefina (Hospital de Sant Joan Despí Moisès Broggi)
Prat i Aymerich, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Latorre, Irene (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)

Data:	2022
Resum:	Current blood-based diagnostic tools for TB are insufficient to properly characterize the distinct stages of TB, from the latent infection (LTBI) to its active form (aTB); nor can they assess treatment efficacy. Several immune cell biomarkers have been proposed as potential candidates for the development of improved diagnostic tools. To compare the capacity of CD27, HLA-DR, CD38 and Ki-67 markers to characterize LTBI, active TB and patients who ended treatment and resolved TB. Blood was collected from 45 patients defined according to clinical and microbiological criteria as: LTBI, aTB with less than 1 month of treatment and aTB after completing treatment. Peripheral blood mononuclear cells were stimulated with ESAT-6/CFP-10 or PPD antigens and acquired for flow cytometry after labelling with conjugated antibodies against CD3, CD4, CD8, CD27, IFN-γ, TNF-α, CD38, HLA-DR, and Ki-67. Conventional and multiparametric analyses were done with FlowJo and OMIQ, respectively. The expression of CD27, CD38, HLA-DR and Ki-67 markers was analyzed in CD4 + T-cells producing IFN-γ and/or TNF-α cytokines after ESAT-6/CFP-10 or PPD stimulation. Within antigen-responsive CD4 + T-cells, CD27 - and CD38 + (ESAT-6/CFP-10-specific), and HLA-DR + and Ki-67 + (PPD- and ESAT-6/CFP-10-specific) populations were significantly increased in aTB compared to LTBI. Ki-67 demonstrated the best discriminative performance as evaluated by ROC analyses (AUC.
Ajuts:	Instituto de Salud Carlos III PI18/00411 Instituto de Salud Carlos III PI19/01408
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Activation markers ; Cluster ; Flow cytometry ; Immune response ; Multiparametric analysis ; Mycobacterium tuberculosis ; T-cells ; Treatment
Publicat a:	Frontiers in microbiology, Vol. 13 (july 2022) , ISSN 1664-302X

DOI: 10.3389/fmicb.2022.885312
PMID: 35935194

12 p, 1.4 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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