Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI
Vilahur, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Sutelman, Pablo (Institut d'Investigació Biomèdica Sant Pau)
Ben-Aicha Gonzalez, Soumaya (Institut d'Investigació Biomèdica Sant Pau)
Mendieta, Guiomar (Institut d'Investigació Biomèdica Sant Pau)
Radiké Aržanauskaitė, Monika (Institut d'Investigació Biomèdica Sant Pau)
Schoch, Leonie (Institut d'Investigació Biomèdica Sant Pau)
Casaní, Laura (Institut d'Investigació Biomèdica Sant Pau)
Borrell-Pages, Maria (Institut d'Investigació Biomèdica Sant Pau)
Padró, Teresa (Institut d'Investigació Biomèdica Sant Pau)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2022
Abstract:	Myocardial infarction (MI) is the clinical manifestation of atherosclerotic coronary artery disease. Spirulina is an algae known to ameliorate cardiometabolic disorders and with proven anti-inflammatory and anti-oxidant effects. We investigated, in a highly translatable animal model, whether oral supplementation with spirulina protects against the deleterious effects triggered by ST-elevation MI (STEMI). Pigs were fed a regular diet supplemented with spirulina (1 g/animal/bid) or placebo-control for 10 days. Thereafter, animals were subjected to 1. 5 h percutaneous balloon-induced coronary occlusion (STEMI) followed by 2. 5 h reperfusion and then sacrificed. We assessed infarct size and cardiac function. Blood samples and infarcted and remote myocardial tissue were obtained. Spirulina supplementation reduced infarct size by 64%, increased myocardial salvage by 18%, and improved cardiac function by 30% vs. controls (p < 0. 05). These benefits were associated with attenuation in DNA-oxidative damage and apoptotic markers and increased iNOS in the infarcted myocardium, higher AMPK activation in the remote myocardium, and lower myocardial MCP-1 expression. Systemically, spirulina attenuated Cox-2 expression in STEMI-activated peripheral blood mononuclear cells and enhanced TNF-α release acutely post-STEMI. Additionally, spirulina decreased weight gain progression over time (p < 0. 05) without changes in lipids, glucose, liver or kidney parameters. A 10-day supplementation with spirulina exerts cardioprotection in a preclinical setting of STEMI by limiting cardiac damage and improving ventricular contractility through anti-oxidative, anti-inflammatory, and anti-apoptotic mechanisms.
Grants:	Agencia Estatal de Investigación PID2019-107160RB-I00 Agencia Estatal de Investigación PGC2018-094025-B-I00 Instituto de Salud Carlos III PI19/01687 Ministerio de Economía y Competitividad CB16/11/00411 Instituto de Salud Carlos III PI19/01687
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Anti-apoptotic ; Anti-inflammatory ; Antioxidation ; Cardioprotection ; Spirulina ; STEMI ; Weight management
Published in:	Frontiers in Pharmacology, Vol. 13 (may 2022) , ISSN 1663-9812

DOI: 10.3389/fphar.2022.891801
PMID: 35592428

13 p, 2.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles