Antimicrobial peptides in the global microbiome : biosynthetic genes and resistance determinants
Chen, Bingfeng (Zhejiang University of Technology. College of Environment)
Zhang, Zhenyan (Zhejiang University of Technology. College of Environment)
Zhang, Qi (Zhejiang University of Technology. College of Environment)
Xu, Nuohan Xu (Zhejiang University of Technology. College of Environment)
Lu, Tao (Zhejiang University of Technology. College of Environment)
Wang, Tingzhang (Key Laboratory of Microbial Technology and Bioinformatics (Zhejiang, Xina))
Hong, Wenjie (Key Laboratory of Microbial Technology and Bioinformatics (Zhejiang, Xina))
Fu, Zhengwei (Zhejiang University of Technology. College of Biotechnology and Bioengineering)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)

Date:	2023
Abstract:	Antimicrobial peptides are a promising new class of antimicrobials that could address the antibiotic resistance crisis, which poses a major threat to human health. These peptides are present in all kingdoms of life, but especially in microorganisms, having multiple origins in diverse taxa. To date, there has been no global study on the diversity of antimicrobial peptides, the hosts in which these occur, and the potential for resistance to these agents. Here, we investigated the diversity and number of antimicrobial peptides in four main habitats (aquatic, terrestrial, human, and engineered) by analyzing 52,515 metagenome-assembled genomes. The number of antimicrobial peptides was higher in the human gut microbiome than in other habitats, and most hosts of antimicrobial peptides were habitat-specific. The relative abundance of genes that confer resistance to antimicrobial peptides varied between habitats and was generally low, except for the built environment and on human skin. The horizontal transfer of potential resistance genes among these habitats was probably constrained by ecological barriers. We systematically quantified the risk of each resistance determinant to human health and found that nearly half of them pose a threat, especially those that confer resistance to multiple AMPs and polymyxin B. Our results help identify the biosynthetic potential of antimicrobial peptides in the global microbiome, further identifying peptides with a low risk of developing resistance.
Grants:	Agencia Estatal de Investigación TED2021-132627B-I00 Agencia Estatal de Investigación PID2019-110521GB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1005
Note:	Altres ajuts: the Fundación Ramón Areces grant CIVP20A6621
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Antibiotic ; Antimicrobial peptide ; Resistance ; Metagenome-assembled genomes ; Health risk ; Biosynthesis
Published in:	Environmental Science & Technology, Vol. 57, issue 20 (2023) , p.7698-7708, ISSN 1520-5851

DOI: 10.1021/acs.est.3c01664

Available from: 2024-12-30 Postprint

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Articles > Research articles
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