Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients
Dortez Herranz, Silvia (Universidad de Alcalá)
De Gregorio-Rocasolano, Nuria (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Millán, Mònica (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gasull Dalmau, Teresa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Crevillen, Agustín G. (Universidad Nacional de Educación a Distancia (Espanya))
Escarpa, Alberto (Universidad de Alcalá)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	For the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously measured on the same PAD using ferrozine as a chromophore and a smartphone as the color reader. To this end, Tf was first isolated from serum using anti-Tf immunomagnetic beads to ensure that only the Tf-bound iron was measured, improving the selectivity and accuracy of TSAT assessment. To demonstrate the practical utility of the device, it was validated by analyzing a certified reference material, showing excellent accuracy (E < 4%) and good precision (RSD ≤ 6%). Finally, 18 diagnosed serum samples from ischemic stroke patients were analyzed by this approach, and the results were compared with those obtained by urea-PAGE, showing not only an excellent correlation (r = 0. 93, p < 0. 05) but that the PAD approach has become statistically identical to the free-interference urea-PAGE. In comparison with the slow, tedious, and non-miniaturized-PAGE, this PAD approach exhibited attractive characteristics such as low cost, disposability, and connectivity, showing great potential for future point-of-care testing, especially in developing countries and/or remote areas, where access to medical or clinical facilities is limited.
Ajuts:	Agencia Estatal de Investigación PID2020-118154GB-I00 Agencia Estatal de Investigación CTQ2017-86441-C2-1-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Color ; Colorimetry ; Iron ; Serum ; Testing and assessment
Publicat a:	Analytical chemistry, Vol. 95 (07 2023) , p. 12391-12397, ISSN 1520-6882

DOI: 10.1021/acs.analchem.3c01982
PMID: 37486019

7 p, 2.8 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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