Conservation of Phenotypes in the Roman High- and Low-Avoidance Rat Strains After Embryo Transfer
Río-Álamos, Cristóbal (Universitat Autònoma de Barcelona. Institut de Neurociències)
Gerbolés, Cristina (Universitat Autònoma de Barcelona. Institut de Neurociències)
Tapias-Espinosa, Carles (Universitat Autònoma de Barcelona. Institut de Neurociències)
Sampedro-Viana, Daniel (Universitat Autònoma de Barcelona. Institut de Neurociències)
Oliveras, Ignasi (Universitat Autònoma de Barcelona. Institut de Neurociències)
Sánchez-González, Ana (Universitat Autònoma de Barcelona. Institut de Neurociències)
Cañete, Toni (Universitat Autònoma de Barcelona. Institut de Neurociències)
Blázquez, Gloria (Universitat Autònoma de Barcelona. Institut de Neurociències)
López, María del Mar (Universitat Autònoma de Barcelona. Servei d'Estabulari (SE))
Baldellou, Carlos (Universitat Autònoma de Barcelona. Servei d'Estabulari (SE))
Otaegui Goya, Pedro José (Universitat Autònoma de Barcelona. Servei d'Estabulari (SE))
Tobeña, Adolf 1950- (Universitat Autònoma de Barcelona. Institut de Neurociències)
Fernández-Teruel, Alberto (Universitat Autònoma de Barcelona. Departament de Psiquiatria i de Medicina Legal)

Date:	2017
Abstract:	The Roman high- (RHA-I) and low-avoidance (RLA-I) rat strains are bi-directionally bred for their good versus non-acquisition of two-way active avoidance, respectively. They have recently been re-derived through embryo transfer (ET) to Sprague-Dawley females to generate specific pathogen free (SPF) RHA-I/RLA-I rats. Offspring were phenotyped at generations 1 (G1, born from Sprague-Dawley females), 3 and 5 (G3 and G5, born from RHA-I and RLA-I from G2-G4, respectively), and compared with generation 60 from our non-SPF colony. Phenotyping included two-way avoidance acquisition, context-conditioned fear, open-field behaviour, novelty-seeking, baseline startle, pre-pulse inhibition (PPI) and stress-induced increase in plasma corticosterone concentration. Post-ET between-strain differences in avoidance acquisition, context-conditioned freezing and novelty-induced self-grooming are conserved. Other behavioural traits (i. e. hole-board head-dipping, novel object exploration, open-field activity, startle, PPI) differentiate the strains at G3-G5 but not at G1, suggesting that the pre-/post-natal environment may have influenced these co-segregated traits at G1, though further selection pressure along the subsequent generations (G1-G5) rescues the typical strain-related differences.
Grants:	Ministerio de Economía y Competitividad PSI2013-41872-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1587
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Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Behavioural phenotyping ; Embryo transfer ; Roman rat strains ; Stress-induced corticosterone ; Two-way active avoidance
Published in:	Behavior Genetics, Vol. 47 N. 5 (2017) , p. 537-555, ISSN 1573-3297

DOI: 10.1007/s10519-017-9854-2

Postprint 58 p, 1.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles