Genomic characterization of enterohaemolysin-encoding haemolytic Escherichia coli of animal and human origin
Sidorczuk, Katarzyna (University of Wrocław)
Aleksandrowicz, Adrianna (Wrocław University of Environmental and Life Sciences)
Burdukiewicz, Michał (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Kingsley, Robert A. (Quadram Institute Biosciences)
Kolenda, Rafał (Quadram Institute Biosciences)

Date:	2023
Abstract:	Enterohaemolysin (Ehx) and alpha-haemolysin are virulence-associated factors (VAFs) causing the haemolytic phenotype in . It has been shown that chromosomally and plasmid-encoded alpha-haemolysin are characteristic of specific pathotypes, virulence-associated factors and hosts. However, the prevalence of alpha- and enterohaemolysin does not overlap in the majority of pathotypes. Therefore, this study focuses on the characterization of the haemolytic population associated with multiple pathotypes in human and animal infectious diseases. Using a genomics approach, we investigated characteristic features of the enterohaemolysin-encoding strains to identify factors differentiating enterohaemolysin-positive from alpha-haemolysin-positive populations. To shed light on the functionality of Ehx subtypes, we analysed Ehx-coding genes and inferred EhxA phylogeny. The two haemolysins are associated with a different repertoire of adhesins, iron acquisition or toxin systems. Alpha-haemolysin is predominantly found in uropathogenic (UPEC) and predicted to be chromosomally encoded, or nonpathogenic and undetermined pathotypes and typically predicted to be plasmid-encoded. Enterohaemolysin is mainly associated with Shiga toxin-producing (STEC) and enterohaemorrhagic (EHEC) and predicted to be plasmid-encoded. Both types of haemolysin are found in atypical enteropathogenic (aEPEC). Moreover, we identified a new EhxA subtype present exclusively in genomes with VAFs characteristic of nonpathogenic . This study reveals a complex relationship between haemolytic of diverse pathotypes, providing a framework for understanding the potential role of haemolysin in pathogenesis.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Escherichia coli ; Haemolytic ; Enterohaemolysin ; Alpha-haemolysin ; Adhesins ; Iron acquisition ; Toxin ; EhxA
Published in:	Microbial Genomics, Vol. 9, Issue 4 (April 2023) , ISSN 2057-5858

DOI: 10.1099/mgen.0.000999
PMID: 37103985

12 p, 4.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles