Activity of Antibiotics and Potential Antibiofilm Agents against Biofilm-Producing Mycobacterium avium-intracellulare Complex Causing Chronic Pulmonary Infections
Portell-Buj, Elena (Hospital Clínic i Provincial de Barcelona)
González-Criollo, Cecibel (Zurita&Zurita Laboratorios)
López-Gavín, Alexandre (Hospital Clínic i Provincial de Barcelona)
Fernández-Pittol, Mariana (Hospital Clínic i Provincial de Barcelona)
Busquets, M. Antònia (Institut Català de Nanociència i Nanotecnologia)
Estelrich i Latràs, Joan (Institut Català de Nanociència i Nanotecnologia)
Garrigó, Montserrat (Institut d'Investigació Biomèdica Sant Pau)
Rubio, Marc (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Tudó, Griselda (Hospital Clínic i Provincial de Barcelona)
Gonzalez-Martin, Julian (Instituto de Salud Carlos III)
Universitat Autònoma de Barcelona

Data:	2022
Resum:	Nontuberculous mycobacteria (NTM) cause lung infections in patients with underlying pulmonary diseases (PD). The Mycobacterium avium-intracellulare complex (MAC) is the most frequently involved NTM. The MAC-PD treatment is based on the administration of several antibiotics for long periods of time. Nonetheless, treatment outcomes remain very poor. Among the factors involved is the ability of MAC isolates to form biofilm. The aim of the study was to assess the in vitro activity of different antibiotics and potential antibiofilm agents (PAAs) against MAC biofilm. Four antibiotics and six PAAs, alone and/or in combination, were tested against planktonic forms of 11 MAC clinical isolates. Biofilm was produced after 4 weeks of incubation and analyzed with the crystal violet assay. The antibiotics and PAAs were tested by measuring the absorbance (minimum biofilm inhibition concentrations, MBICs) and by performing subcultures (minimum biofilm eradication concentrations, MBECs). The clarithromycin/amikacin and clarithromycin/ethambutol combinations were synergistic, decreasing the MBECs values compared to the individual antibiotics. The amikacin/moxifloxacin combination showed indifference. The MBIC values decreased significantly when PAAs were added to the antibiotic combinations. These results suggest that antibiotic combinations should be further studied to establish their antibiofilm activity. Moreover, PAAs could act against the biofilm matrix, facilitating the activity of antibiotics.
Ajuts:	Ministerio de Economía y Competitividad PI16/01047
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Antibiotic combinations ; Biofilm ; Minimum biofilm eradication concentrations ; Minimum biofilm inhibitory concentrations ; Nontuberculous mycobacteria ; Potential antibiofilm agents
Publicat a:	Antibiotics, Vol. 11 Núm. 5 (may 2022) , p. 589, ISSN 2079-6382

DOI: 10.3390/antibiotics11050589
PMID: 35625233

10 p, 611.7 KB

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