Early Macrophage Infiltration and Sustained Inflammation in Kidneys From Deceased Donors Are Associated With Long-Term Renal Function
Guillén-Gómez, E. (Institut d'Investigació Biomèdica Sant Pau)
Dasilva, I. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Silva, Irene (Institut d'Investigació Biomèdica Sant Pau)
Arce, Yolanda (Institut d'Investigació Biomèdica Sant Pau)
Facundo, Carme (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ars, Elisabet (Institut d'Investigació Biomèdica Sant Pau)
Breda, Alberto (Institut d'Investigació Biomèdica Sant Pau)
Ortiz, Alberto (Instituto de Investigación Sanitaria de la Fundación Jiménez Díaz)
Guirado, Luis (Institut d'Investigació Biomèdica Sant Pau)
Ballarín Castan, José Aurelio (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Díaz Encarnación, Montserrat Mercedes (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2017
Abstract:	Kidney transplants from living donors (LDs) have a better outcome than those from deceased donors (DDs). Different factors have been suggested to justify the different outcome. In this study, we analyzed the infiltration and phenotype of monocytes/macrophages and the expression of inflammatory and fibrotic markers in renal biopsy specimens from 94 kidney recipients (60 DDs and 34 LDs) at baseline and 4 months after transplantation. We evaluated their association with medium- and long-term renal function. At baseline, inflammatory gene expression was higher in DDs than in LDs. These results were confirmed by the high number of CD68-positive cells in DD kidneys, which correlated negatively with long-term renal function. Expression of the fibrotic markers vimentin, fibronectin, and α-smooth muscle actin was more elevated in biopsy specimens from DDs at 4 months than in those from LDs. Gene expression of inflammatory and fibrotic markers at 4 months and difference between 4 months and baseline correlated negatively with medium- and long-term renal function in DDs. Multivariate analysis point to transforming growth factor-β1 as the best predictor of long-term renal function in DDs. We conclude that early macrophage infiltration, sustained inflammation, and transforming growth factor-β1 expression, at least for the first 4 months, contribute significantly to the difference in DD and LD transplant outcome.
Grants:	Ministerio de Economía y Competitividad RD12/0021/0033 Ministerio de Economía y Competitividad PI12/01524
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Basic (laboratory) research/science ; Biopsy ; Donors and donation ; Fibrosis ; Graft survival ; Kidney (allograft) function/dysfunction ; Kidney transplantation/nephrology ; Molecular biology ; Translational research/science
Published in:	American Journal of Transplantation, Vol. 17 Núm. 3 (january 2017) , p. 733-743, ISSN 1600-6143

DOI: 10.1111/ajt.13998
PMID: 27496082

11 p, 338.4 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles