Investigating the Role of Brain Natriuretic Peptide (BNP) and N-Terminal-proBNP in Thrombosis and Acute Ischemic Stroke Etiology
Rossi, Rosanna (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Jabrah, Duaa (University of Galway)
Douglas, Andrew (University of Galway)
Prendergast, James (University of Galway)
Pandit, Abhay (University of Galway)
Gilvarry, Michael (Corporate House)
McCarthy, Ray (Corporate House)
Redfors, Petra (University of Gothenburg)
Nordanstig, Annika (University of Gothenburg)
Tatlisumak, Turgut (University of Gothenburg)
Ceder, Erik (Sahlgrenska University Hospital (Suècia))
Dunker, Dennis (Sahlgrenska University Hospital (Suècia))
Carlqvist, Jeanette (Sahlgrenska University Hospital (Suècia))
Szikora, István (National Institute of Clinical Neurosciences)
Tsivgoulis, Georgios (National & Kapodistrian University of Athens)
Psychogios, Klearchos (Metropolitan Hospital)
Thornton, John (Beaumont Hospital (Dublín, Irlanda))
Rentzos, Alexandros (Sahlgrenska University Hospital (Suècia))
Jood, Katarina (Sahlgrenska University Hospital (Suècia))
Juega, Jesus (Hospital Universitari Vall d'Hebron)
Doyle, Karen M. (University of Galway)

Data:	2024
Resum:	The need for biomarkers for acute ischemic stroke (AIS) to understand the mechanisms implicated in pathological clot formation is critical. The levels of the brain natriuretic peptides known as brain natriuretic peptide (BNP) and NT-proBNP have been shown to be increased in patients suffering from heart failure and other heart conditions. We measured their expression in AIS clots of cardioembolic (CE) and large artery atherosclerosis (LAA) etiology, evaluating their location inside the clots, aiming to uncover their possible role in thrombosis. We analyzed 80 thrombi from 80 AIS patients in the RESTORE registry of AIS clots, 40 of which were of CE and 40 of LAA etiology. The localization of BNP and NT-BNP, quantified using immunohistochemistry and immunofluorescence, in AIS-associated white blood cell subtypes was also investigated. We found a statistically significant positive correlation between BNP and NT-proBNP expression levels (Spearman's rho = 0. 668 p < 0. 0001 *). We did not observe any statistically significant difference between LAA and CE clots in BNP expression (0. 66 [0. 13-3. 54]% vs. 0. 53 [0. 14-3. 07]%, p = 0. 923) or in NT-proBNP expression (0. 29 [0. 11-0. 58]% vs. 0. 18 [0. 05-0. 51]%, p = 0. 119), although there was a trend of higher NT-proBNP expression in the LAA clots. It was noticeable that BNP was distributed throughout the thrombus and especially within platelet-rich regions. However, NT-proBNP colocalized with neutrophils, macrophages, and T-lymphocytes, suggesting its association with the thrombo-inflammatory process.
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Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Brain natriuretic peptide ; BNP ; NT-proBNP ; Stroke biomarkers ; Thrombus ; Acute ischemic stroke ; Stroke etiology
Publicat a:	International journal of molecular sciences, Vol. 25, Issue 5 (March 2024) , art. 2999, ISSN 1422-0067

DOI: 10.3390/ijms25052999
PMID: 38474245

12 p, 6.6 MB

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