Thimet oligopeptidase as a potential CSF biomarker for Alzheimer's disease : A cross-platform validation study
Hok-A-Hin, Yanaika S. (Amsterdam University Medical Center (UMC))
Bolsewig, Katharina (Amsterdam University Medical Center (UMC))
Ruiters, Daimy N. (Amsterdam University Medical Center (UMC))
Lleó, Alberto (Institut d'Investigació Biomèdica Sant Pau)
Alcolea, Daniel (Institut d'Investigació Biomèdica Sant Pau)
Lemstra, Afina W. (Vrije Universiteit Amsterdam)
van der Flier, Wiesje M. (Amsterdam University Medical Center (UMC))
Teunissen, Charlotte E. (Amsterdam University Medical Center (UMC))
Campo Milán, Marta del (Fundació Pasqual Maragall)
Universitat Autònoma de Barcelona

Date:	2023
Abstract:	Our previous antibody-based cerebrospinal fluid (CSF) proteomics study showed that Thimet oligopeptidase (THOP1), an amyloid beta (Aβ) neuropeptidase, was increased in mild cognitive impairment with amyloid pathology (MCI-Aβ+) and Alzheimer's disease (AD) dementia compared with controls and dementia with Lewy bodies (DLB), highlighting the potential of CSF THOP1 as an early specific biomarker for AD. We aimed to develop THOP1 immunoassays for large-scale analysis and validate our proteomics findings in two independent cohorts. We developed in-house CSF THOP1 immunoassays on automated Ella and Simoa platforms. The performance of the different assays were compared using Passing-Bablok regression analysis in a subset of CSF samples from the discovery cohort (n = 72). Clinical validation was performed in two independent cohorts (cohort 1: n = 200; cohort 2: n = 165) using the Ella platform. THOP1 concentrations moderately correlated between proteomics analysis and our novel assays (Rho.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Alzheimer's disease ; Biomarkers ; CSF ; THOP1
Published in:	Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, Vol. 15 (july 2023) , ISSN 2352-8729

DOI: 10.1002/dad2.12456
PMID: 37502019

11 p, 2.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles