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Harmful effects of true-to-life nanoplastics derived from PET water bottles in human alveolar macrophages
Tavakolpournegari, Alireza (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villacorta, Aliro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Morataya-Reyes, Michelle (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Arribas Arranz, Jéssica (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Banaei, Gooya (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Pastor Benito, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Velázquez, Antonia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Annangi, Balasubramanyam (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data: 2024
Resum: The increasing presence of secondary micro/nanoplastics (MNPLs) in the environment requires knowing if they represent a real health concern. To such end, an important point is to test representative MNPLs such as the denominated true-to-life MNPLs, resulting from the degradation of plastic goods in lab conditions. In this study, we have used polyethylene terephthalate (PET) NPLs resulting from the degradation of PET water bottles. Since inhalation is an important exposure route to environmental MNPLS, we have used mouse alveolar macrophages (MH-S) as a target cell, and the study focused only on the cells that have internalized them. This type of approach is novel as it may capture the realistic adverse effects of PETNPLs only in the internalized cells, thereby mitigating any biases while assessing the risk of these MNPLs. Furthermore, the study utilized a set of biomarkers including intracellular reactive oxygen species (ROS) levels, variations on the mitochondrial membrane potential values, and the macrophage polarization to M1 (pro-inflammatory response) and M2 (anti-proinflammatory response) as possible cellular effects due to PETNPLs in only the cells that internalized PETNPLs. After exposures lasting for 3 and 24 h to a range of concentrations (0, 25, 50, and 100 μg/mL) the results indicate that no toxicity was induced despite the 100% internalization observed at the highest concentration. Significant intracellular levels of ROS were observed, mainly at exposures lasting for 24 h, in an indirect concentration-effect relationship. Interestingly, a reduction in the mitochondrial membrane potential was observed, but only at exposures lasting for 24 h, but without a clear concentration-effect relationship. Finally, PETNPL exposure shows a significant polarization from M0 to M1 and M2 subtypes. Polarization to M1 (pro-inflammatory stage) was more marked and occurred at both exposure times. Polarization to M2 (anti-inflammatory stage) was only observed after exposures lasting for 24 h. Due to the relevance of the described biomarkers, our results underscore the need for further research, to better understand the health implications associated with MNPL exposure.
Ajuts: European Commission 965196
Agencia Estatal de Investigación PID2020-116789RB-C43
Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731
Nota: Altres ajuts: acords transformatius de la UAB
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: MH-S cells ; Macrophage polarization ; Mitochondrial membrane potential ; PET ; ROS
Publicat a: Environmental Pollution, Vol. 348 (May 2024) , art. 123823, ISSN 1873-6424

DOI: 10.1016/j.envpol.2024.123823
PMID: 38513942


12 p, 3.6 MB

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 Registre creat el 2024-05-03, darrera modificació el 2024-05-13



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