Temporal and sex-dependent gene expression patterns in a renal ischemia-reperfusion injury and recovery pig model
Nemours, Stéphane (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Castro, Luis (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ribatallada-Soriano, Didac (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Semidey Raven, Maria Eugenia (Hospital Universitari Vall d'Hebron)
Aranda Martín, Miguel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ferrer, Marina (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Sánchez, Alex (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Morote, Joan (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Cantero-Recasens, Gerard (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Meseguer Navarro, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)

Data:	2022
Resum:	Men are more prone to acute kidney injury (AKI) and chronic kidney disease (CKD), progressing to end-stage renal disease (ESRD) than women. Severity and capacity to regenerate after AKI are important determinants of CKD progression, and of patient morbidity and mortality in the hospital setting. To determine sex differences during injury and recovery we have generated a female and male renal ischemia/reperfusion injury (IRI) pig model, which represents a major cause of AKI. Although no differences were found in blood urea nitrogen (BUN) and serum creatinine (SCr) levels between both sexes, females exhibited higher mononuclear infiltrates at basal and recovery, while males showed more tubular damage at injury. Global transcriptomic analyses of kidney biopsies from our IRI pig model revealed a sexual dimorphism in the temporal regulation of genes and pathways relevant for kidney injury and repair, which was also detected in human samples. Enrichment analysis of gene sets revealed five temporal and four sexual patterns governing renal IRI and recovery. Overall, this study constitutes an extensive characterization of the time and sex differences occurring during renal IRI and recovery at gene expression level and offers a template of translational value for further study of sexual dimorphism in kidney diseases.
Ajuts:	Ministerio de Economía y Competitividad SAF2014-59945-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Kidney ; Mechanisms of disease ; Microarrays
Publicat a:	Scientific reports, Vol. 12 (april 2022) , ISSN 2045-2322

DOI: 10.1038/s41598-022-10352-3
PMID: 35484379

22 p, 4.7 MB

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