Self-sampling monkeypox virus testing in high-risk populations, asymptomatic or with unrecognized Mpox, in Spain
Agustí, Cristina (Institut Germans Trias i Pujol)
Martínez Riveros, Héctor (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i de Medicina Preventiva i Salut Pública)
Hernández-Rodríguez, Àgueda (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Casañ, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Díaz, Yesika (Centre of epidemiological studies on sexually transmitted infections and AIDS of Catalunya)
Alonso García, Lucía (Institut Germans Trias i Pujol. Fundació Lluita Contra les Infeccions)
Martró, Elisa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Muñoz-Basagoiti, Jordana (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Gallemí, Marçal (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Folch, Cinta (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i de Medicina Preventiva i Salut Pública)
Sönmez, Ibrahim (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i de Medicina Preventiva i Salut Pública)
Adell, Héctor (ONG Stop (Barcelona))
Villar, Marta (ONG Stop (Barcelona))
París de León, Alexia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Martinez-Puchol, Sandra (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pelegrin, A. C. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Perez-Zsolt, Daniel (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Raïch-Regué, Dàlia (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Mora, Rubén (ONG Stop (Barcelona))
Villegas, Luis (ONG Stop (Barcelona))
Clotet Sala, Bonaventura (Institut Germans Trias i Pujol. Fundació Lluita Contra les Infeccions)
Izquierdo Useros, Nuria (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Cardona, Pere-Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Casabona-Barbarà, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2023
Abstract:	The recent monkeypox virus (MPXV) outbreak was of global concern and has mainly affected gay, bisexual and other men who have sex with men (GBMSM). Here we assess prevalence of MPXV in high-risk populations of GBMSM, trans women (TW) and non-binary people without symptoms or with unrecognized monkeypox (Mpox) symptoms, using a self-sampling strategy. Anal and pharyngeal swabs are tested by MPXV real-time PCR and positive samples are tested for cytopathic effect (CPE) in cell culture. 113 individuals participated in the study, 89 (78. 76%) were cis men, 17 (15. 04%) were TW. The median age was 35. 0 years (IQR: 30. 0-43. 0), 96 (85. 02%) individuals were gay or bisexual and 72 (63. 72%) were migrants. Seven participants were MPXV positive (6. 19% (95% CI: 1. 75%-10. 64%)). Five tested positive in pharyngeal swabs, one in anal swab and one in both. Six did not present symptoms recognized as MPXV infection. Three samples were positive for CPE, and showed anti-vaccinia pAb staining by FACS and confocal microscopy. This suggests that unrecognized Mpox cases can shed infectious virus. Restricting testing to individuals reporting Mpox symptoms may not be sufficient to contain outbreaks. The monkeypox outbreak in 2022 was of global concern. Here the authors present results from an observational study providing self-sampling testing to a high-risk population in a community center in Spain and diagnose monkeypox infection in asymptomatic individuals.
Grants:	Agencia Estatal de Investigación PID2020-117145RB-I00 European Commission 10104611
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Epidemiology ; Viral infection
Published in:	Nature communications, Vol. 14 (october 2023) , ISSN 2041-1723

DOI: 10.1038/s41467-023-40490-9
PMID: 37783731

11 p, 902.5 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles