Using the electronic nose to identify airway infection during COPD exacerbations
Shafiek, Hanaa (Hospital Universitari Son Espases (Palma de Mallorca, Balears))
Fiorentino, Federico (Hospital Universitari Son Espases (Palma de Mallorca, Balears))
Merino, José Luis (Universitat de les Illes Balears)
López, Carla (Hospital Universitari Son Espases (Palma de Mallorca, Balears))
Oliver, Antonio (Hospital Universitari Son Espases (Palma de Mallorca, Balears))
Segura, Jaume (Universitat de les Illes Balears)
De Paul, Ivan (Universitat de les Illes Balears)
Sibila, Oriol (Institut d'Investigació Biomèdica Sant Pau)
Agustí, Àlvar (Universitat de Barcelona)
Cosío, Borja G. (Hospital Universitari Son Espases (Palma de Mallorca, Balears))
Universitat Autònoma de Barcelona

Data:	2015
Resum:	Background: The electronic nose (e-nose) detects volatile organic compounds (VOCs) in exhaled air. We hypothesized that the exhaled VOCs print is different in stable vs. exacerbated patients with chronic obstructive pulmonary disease (COPD), particularly if the latter is associated with airway bacterial infection, and that the e-nose can distinguish them. Methods: Smell-prints of the bacteria most commonly involved in exacerbations of COPD (ECOPD) were identified in vitro. Subsequently, we tested our hypothesis in 93 patients with ECOPD, 19 of them with pneumonia, 50 with stable COPD and 30 healthy controls in a cross-sectional case-controlled study. Secondly, ECOPD patients were re-studied after 2 months if clinically stable. Exhaled air was collected within a Tedlar bag and processed by a Cynarose 320 e-nose. Breath-prints were analyzed by Linear Discriminant Analysis (LDA) with "One Out" technique and Sensor logic Relations (SLR). Sputum samples were collected for culture. Results: ECOPD with evidence of infection were significantly distinguishable from non-infected ECOPD (p = 0. 018), with better accuracy when ECOPD was associated to pneumonia. The same patients with ECOPD were significantly distinguishable from stable COPD during followup (p = 0. 018), unless the patient was colonized. Additionally, breath-prints from COPD patients were significantly distinguished from healthy controls. Various bacteria species were identified in culture but the e-nose was unable to identify accurately the bacteria smellprint in infected patients. Conclusion: E-nose can identify ECOPD, especially if associated with airway bacterial infection or pneumonia.
Ajuts:	Ministerio de Economía y Competitividad PI12/02628
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Aged ; Bacteria ; Bacterial Infections ; Breath Tests ; Case-Control Studies ; Cross-Sectional Studies ; Electronic Nose ; Equipment Design ; Exhalation ; Female ; Humans ; Lung ; Male ; Middle Aged ; Pneumonia ; Pulmonary Disease, Chronic Obstructive ; Volatile Organic Compounds
Publicat a:	PloS one, Vol. 10 Núm. 9 (september 2015) , p. e0135199, ISSN 1932-6203

DOI: 10.1371/journal.pone.0135199
PMID: 26353114

16 p, 1.9 MB

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