Lipidomic changes of LDL in overweight and moderately hypercholesterolemic subjects taking phytosterol- and omega-3-supplemented milk
Padró, Teresa (Institut d'Investigació Biomèdica Sant Pau)
Vilahur, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Sánchez-Hernández, Joan (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Hernández, Marta (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Antonijoan Arbós, Rosa Ma (Rosa María) (Institut d'Investigació Biomèdica Sant Pau)
Pérez Pérez, Antonio (Institut d'Investigació Biomèdica Sant Pau)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia

Date:	2015
Abstract:	The benefits of dietary phytosterols (PhySs) and long-chain n-3 PUFA (ω3) have been linked to their effects as cholesterol- and triglyceride (TGL)-lowering agents. However, it remains unknown whether these compounds have further metabolic effects on LDL lipid composition. Here, we studied the effects of PhyS- or ω3-supplemented low-fat milk (milk) on the LDL-lipidome. Overweight and moderately hypercholesterolemic subjects (n = 32) were enrolled in a two-arm longitudinal crossover study. Milk (250 ml/day), enriched with either 1. 57 g PhyS or 375 mg ω3 (EPA + DHA), was given to the participants during two sequential 28 day intervention periods. Compared with baseline, PhyS-milk induced a higher reduction in the LDL cholesterol (LDLc) level than ω3-milk. LDL resistance to oxidation was significantly increased after intervention with PhyS-milk. Changes in TGL and VLDL cholesterol were only evident after ω3-milk intake. Lipidomic analysis revealed a differential effect of the PhyS- and ω3-milk interventions on the LDL lipid metabolite pattern. Content in LDL-glycerophospholipids was reduced after PhyS-milk intake, with major changes in phosphatidylcholine (PC) and phosphatidylserine subclasses, whereas ω3-milk induced significant changes in the long-chain polyunsaturated cholesteryl esters and in the ratio PC36:5/lysoPC16:0, associated to a reduced inflammatory activity. In conclusion, daily intake of milk products containing PhyS or ω3 supplements induce changes in the LDL-lipidome that indicate reduced inflammatory and atherogenic effects, beyond their LDLc- and TGL-lowering effects.
Grants:	Ministerio de Ciencia e Innovación CEN-20101016 Ministerio de Economía y Competitividad SAF2013-42962-R Ministerio de Economía y Competitividad SAF2012-40208
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Atherosclerosis ; Cholesterol ; Diet and dietary lipids ; Glycerophospholipids ; Low density lipoprotein-lipid metabolites ; Mass spectrometry ; Phospholipids ; Triglycerides
Published in:	Journal of Lipid Research, Vol. 56 Núm. 5 (january 2015) , p. 1043-1056, ISSN 1539-7262

DOI: 10.1194/jlr.P052217
PMID: 25773888

14 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles