Impact of diesel exhaust particles on infections with Mycobacterium bovis BCG in in vitro human macrophages and an in vivo Galleria mellonella model
Rodríguez-Fernández, Pablo (University of Manchester)
Romero i Andrada, Iris (Institut Germans Trias i Pujol)
Molina-Moya, Barbara (Institut Germans Trias i Pujol)
Latorre, Irene (Institut Germans Trias i Pujol)
Lacoma, Alicia (Institut Germans Trias i Pujol)
Prat i Aymerich, Cristina (Institut Germans Trias i Pujol)
Tabernero, Lydia (University of Manchester)
Domínguez, José (Institut Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Data:	2024
Resum:	There are strong suggestions for a link between pulmonary tuberculosis (TB) and air quality. Diesel exhaust is one of the main contributors to pollution and it is reported to be able to modify susceptibility to lung infections. In this study we exposed THP-1 human macrophages and Mycobacterium bovis BCG to diesel exhaust particles (DEPs). High cytotoxicity and activation of apoptosis was found in THP-1 cells at 3 and 6 days, but no effect was found on the growth of M. bovis BCG. Infection of THP-1 cells exposed to a non-cytotoxic DEP concentration showed a limited capacity to engulf latex beads. However, M. bovis BCG infection of macrophages did not result in an increase in the bacterial burden, but it did result in an increase in the bacteria recovered from the extracellular media, suggesting a poor contention of M. bovis BCG. We also observed that DEP exposure limited the production of cytokines. Using the Galleria mellonella model of infection, we observed that larvae exposed to low levels of DEPs were less able to survive after infection with M. bovis BCG and had a higher internal bacterial load after 4 days of infection. Unraveling the links between air pollution and impairment of human antimycobacterial immunity is vital, because pollution is rapidly increasing in areas where TB incidence is extremely high.
Ajuts:	Instituto de Salud Carlos III PI19/01408 European Commission 823854
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Environmental pollution, Vol. 341 (15 2024) , p. 122597, ISSN 1873-6424

DOI: 10.1016/j.envpol.2023.122597
PMID: 37741543

11 p, 5.1 MB

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