Decoding CD4+ T cell transcriptome in giant cell arteritis : Novel pathways and altered cross-talk with monocytes
Estupiñán-Moreno, Elkyn (Instituto de Parasitología y Biomedicina López Neyra)
Hernández-Rodríguez, José (Institut D'Investigacions Biomèdiques August Pi I Sunyer)
Li, Tianlu (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Ciudad, Laura (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Andrés-León, Eduardo (Instituto de Parasitología y Biomedicina López Neyra)
Terron-Camero, Laura Carmen (Instituto de Parasitología y Biomedicina López Neyra)
Prieto-González, Sergio (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Espígol-Frigolé, Georgina (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Cid, Maria C. (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Márquez, Ana (Instituto de Parasitología y Biomedicina López Neyra)
Martin, Javier (Instituto de Parasitología y Biomedicina López Neyra)
Ballestar, Esteban (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Ortiz-Fernández, Lourdes (Instituto de Parasitología y Biomedicina López Neyra)

Data:	2024
Resum:	Background: Giant cell arteritis (GCA) is an immune-mediated large-vessels vasculitis with complex etiology. Although the pathogenic mechanisms remain poorly understood, a central role for CD4 T cells has been demonstrated. In this context, understanding the transcriptome dysregulation in GCA CD4 T cells will yield new insights into its pathogenesis. Methods: Transcriptome analysis was conducted on CD4 T cells from 70 patients with GCA with different disease activity and treatment status (active patients before treatment and patients in remission with and without glucocorticoid treatment), and 28 healthy controls. The study also evaluated potential impacts of DNA methylation on gene expression alterations and assessed cross-talk with CD14 monocytes. Results: This study has uncovered a substantial number of genes and pathways potentially contributing to the pathogenicity of CD4 T cells in GCA. Specifically, CD4 T cells from GCA patients with active disease exhibited altered expression levels of genes involved in multiple immune-related processes, including various interleukins (IL) signaling pathways. Notably, IL-2, a decisive interleukin for regulatory T cells homeostasis, was among the most significant. Additionally, impaired apoptotic pathways appear crucial in GCA development. Our findings also suggest that histone-related epigenetic pathways may be implicated in promoting an inflammatory phenotype in GCA active patients. Finally, our study observed altered signaling communication, such as the Jagged-Notch signaling, between CD4 T cells and monocytes that could have pathogenic relevance in GCA. Conclusions: Our study suggests the participation of novel cytokines and pathways and the occurrence of a disruption of monocyte-T cell crosstalk driving GCA pathogenesis.
Ajuts:	European Commission 813545
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Journal of autoimmunity, Vol. 146 (june 2024) , p. 103240, ISSN 1095-9157

DOI: 10.1016/j.jaut.2024.103240

11 p, 6.7 MB

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