Development and Evaluation of an NTM-IGRA to Guide Pediatric Lymphadenitis Diagnosis
Villar-Hernández, Raquel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Latorre, Irene (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Noguera-Julian, Antoni (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Martínez-Planas, Aina (Red de Investigación Translacional en Infectología Pediátrica (RITIP))
Minguell, Laura (Hospital Universitari Arnau de Vilanova)
Vallmanya, Teresa (Hospital Universitari Arnau de Vilanova)
Méndez Hernández, Maria (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Soriano-Arandes, Antoni (Hospital Universitari Vall d'Hebron)
Baquero-Artigao, Fernando (Instituto de Salud Carlos III)
Rodríguez-Molino, Paula (Instituto de Salud Carlos III)
Guillén-Martín, Sara (Hospital Universitario de Getafe (Madrid))
Toro-Rueda, Carlos (Hospital Universitario La Paz (Madrid))
De Souza Galvão, Maria Luiza (Hospital Universitari Vall d'Hebron)
Jiménez-Fuentes, M.Ángeles (Hospital Universitari Vall d'Hebron)
Stojanovic, Zoran (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sabriá, Josefina (Hospital de Sant Joan Despí Moisès Broggi)
Santos, José Ramón (Institut Germans Trias i Pujol. Fundació Lluita Contra les Infeccions)
Puig, Jordi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Domínguez-Álvarez, Marisol (Unidad Clínica de Tratamiento Directamente Observado Serveis Clinics)
Millet, Joan Pau (Hospital General de Granollers)
Altet, Neus (Hospital General de Granollers)
Galea, Yolanda (Utrecht University)
Muriel-Moreno, Beatriz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
García-García, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Bach Griera, Marc (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Prat-Aymerich, Cristina (Hospital General de Granollers)
Julián, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Torrelles, Jordi B. (Texas Biomedical Research Institute)
Rodrigo Gonzalo de Líria, Carlos (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Domínguez, José (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2024
Abstract:	Background: Diagnosis of nontuberculous mycobacteria (NTM) infections remains a challenge. In this study, we describe the evaluation of an immunological NTM-interferon (IFN)-γ release assay (IGRA) that we developed using glycopeptidolipids (GPLs) as NTM-specific antigens. Methods: We tested the NTM-IGRA in 99 samples from pediatric patients. Seventy-five were patients with lymphadenitis: 25 were NTM confirmed, 45 were of unknown etiology but compatible with mycobacterial infection and 5 had lymphadenitis caused by an etiologic agent other than NTM. The remaining 24 samples were from control individuals without lymphadenitis (latently infected with M. Tuberculosis, uninfected controls and active tuberculosis patients). Peripheral blood mononuclear cells were stimulated overnight with GPLs. Detection of IFN-γ producing cells was evaluated by enzyme-linked immunospot assay. Results: NTM culture-confirmed lymphadenitis patient samples had a significantly higher response to GPLs than the patients with lymphadenitis of unknown etiology but compatible with mycobacterial infection (P < 0. 001) and lymphadenitis not caused by NTM (P < 0. 01). We analyzed the response against GPLs in samples from unknown etiology lymphadenitis but compatible with mycobacterial infection cases according to the tuberculin skin test (TST) response, and although not statistically significant, those with a TST ≥5 mm had a higher response to GPLs when compared with the TST <5 mm group. Conclusions: Stimulation with GPLs yielded promising results in detecting NTM infection in pediatric patients with lymphadenitis. Our results indicate that the test could be useful to guide the diagnosis of pediatric lymphadenitis. This new NTM-IGRA could improve the clinical handling of NTM-infected patients and avoid unnecessary misdiagnosis and treatments.
Grants:	Fundació la Marató de TV3 202134-30-31 Ministerio de Economía y Competitividad PI13/01546 Ministerio de Economía y Competitividad PI16/01912 Instituto de Salud Carlos III PI19/01408 Instituto de Salud Carlos III PI22/00766 Instituto de Salud Carlos III PI18/0041 "la Caixa" Foundation CP20/00070 Generalitat de Catalunya 25/2016 Generalitat de Catalunya 691/2018 Generalitat de Catalunya 169/2022 European Commission 823854 "la Caixa" Foundation CI16/00047
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Nontuberculous mycobacteria ; Tuberculosis ; Interferon-γ ; Release assay ; Glycopeptidolipids ; Enzyme-linked immunospot assay
Published in:	The Pediatric infectious disease journal, Vol. 43 Núm. 3 (january 2024) , p. 278-285, ISSN 1532-0987

DOI: 10.1097/INF.0000000000004211

8 p, 588.9 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles