Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
Prat-Vidal, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Crisóstomo, Verónica (Instituto de Salud Carlos III)
Moscoso, Isabel (Instituto de Salud Carlos III)
Báez-Díaz, Claudia (Instituto de Salud Carlos III)
Blanco-Blázquez, Virginia (Instituto de Salud Carlos III)
Gómez-Mauricio, Guadalupe (Jesús Usón Minimally Invasive Surgery Center)
Albericio, Guillermo (Immunology and Oncology Department, National Center for Biotechnology)
Aguilar, Susana (Immunology and Oncology Department, National Center for Biotechnology)
Fernández-Santos, María-Eugenia (Instituto de Salud Carlos III)
Fernández Avilés, F (Instituto de Salud Carlos III)
Sánchez Margallo, Francisco Miguel (Instituto de Salud Carlos III)
Bayés-Genís, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)
Bernad, Antonio (Immunology and Oncology Department, National Center for Biotechnology)

Date:	2021
Abstract:	Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFP pos and HGF-mCherry pos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 10 6 /animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function.
Grants:	Ministerio de Economía y Competitividad RD16/0011/0037 Ministerio de Economía y Competitividad RD16/0011/0006 Ministerio de Economía y Competitividad CB16/11/00403 Ministerio de Economía y Competitividad CB16/11/00494 Agencia Estatal de Investigación RTI2018-097604-B-I00 Agencia Estatal de Investigación SAF2017-84324-C2-1-R Instituto de Salud Carlos III PIC18/00014 Instituto de Salud Carlos III PI20/00247
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cardiac progenitor cell ; IGF-1 ; HGF ; Lentiviral vectors ; Decellularized extracellular matrix ; Porcine large animal model ; Myocardial infarction
Published in:	Cells, Vol. 10, Num. 10 (september 2021) , ISSN 2073-4409

DOI: 10.3390/cells10102571
PMID: 34685551

25 p, 4.6 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles