Candida albicans infection model in Drosophila melanogaster suggests a strain-specific virulent factor boosting a stormy innate immune response
Cortacans, Mariona (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Arch Sisquella, Marta (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Fuentes, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Cardona, Pere-Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2024
Resum:	Pathogens drive the evolution of host defence strategies, with both innate and adaptive immune systems playing key roles. Priming enhances the innate immune system's readiness by functionally reprogramming immune cells after initial exposure to stimuli, like β-glucans. In this sense, Drosophila melanogaster is a valuable model to evaluate the role of innate immunity to control infections. In this study we aimed to set light on the immune priming effect of oral treatment with heat-killed M. manresensis and two different heat-killed C. albicans isolates upon systemic infection by C. albicans in the D. melanogaster model. A clinical and a control ATCC 90028 Candida albicans strain were used. Flies were primed through oral administration of heat-killed C. albicans (hkCa), both clinical and control, and hk- Mycolicibacterium manresensis. After priming, flies were systemically infected with both C. albicans isolates. Host survival, pathogen load, and immune response in response to treatment and infection were evaluated. Both treatments showed a significant capacity to enhance the expression of antimicrobial peptides, in particular Diptericin, and Drosomycin in males. This response had a marked sexual dimorphism due to the difference in Upd3, Nox, and Duox expression. Surprisingly, even when priming was able to avoid the growth of both C. albicans strains, survival was not improved in the case of the clinical isolate, causing an unexpected mortality rate in hours, regardless of the host's sex. Gene expression analysis 24 hours post-infection showed an exacerbated increase in Diptericin, Drosomycin and Upd3 expression upon infection with the clinical strain. Data herein suggests the presence of a strain-specific component in C. albicans as the booster of a "stormy" innate immune response, which must be further investigated, and position D. melanogaster as a useful model for evaluating virulent factors related to the modulation of the innate immunity.
Ajuts:	"la Caixa" Foundation ID471100010434 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-0931472 Instituto de Salud Carlos III PI20/01431 "la Caixa" Foundation LCF/PR/GN16/10290002
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Priming ; Innate immunity ; Drosophila melanogaster ; Infection ; Sexual dimorphism ; Pathogen load ; Β-glucan ; Environmental mycobacteria
Publicat a:	Frontiers in immunology, Vol. 15 (october 2024) , ISSN 1664-3224

DOI: 10.3389/fimmu.2024.1474516
PMID: 39544927

13 p, 5.8 MB

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