Retrospective Analyses of Porcine Circovirus Type 3 (PCV-3) in Switzerland
Rosato, Giuliana (University of Zurich)
Makoni, Grace Makanaka (University of Zurich)
Cobos, Àlex (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Sibila Vidal, Rosa Marina (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Marti, Hanna (University of Zurich)
Prähauser, Barbara (University of Zurich)
Seehusen, Frauke (University of Zurich)

Date:	2024
Abstract:	Porcine circovirus 3 (PCV-3) has emerged as a significant pathogen affecting global swine populations, yet its epidemiology and clinical implications remain incompletely understood. This retrospective study aimed to investigate the prevalence and histopathological features of PCV-3 infection in pigs from Switzerland, focusing on archival cases of suckling and weaner piglets presenting with suggestive lesions. An in-house qPCR assay was developed for detecting PCV-3 in frozen and formalin-fixed paraffin-embedded tissues, enhancing the national diagnostic capabilities. Histopathological reassessment identified PCV-3 systemic disease (PCV-3-SD) compatible lesions in 19 (6%) of archival cases, with 47% testing positive by qPCR across various organs. Notably, vascular lesions predominated, particularly in mesenteric arteries, heart, and kidneys. The study confirms the presence of PCV-3 in Switzerland since at least 2020, marking the first documented cases within the Swiss swine population. Despite challenges in in situ hybridization validation due to prolonged formalin fixation, the findings indicate viral systemic dissemination. These results contribute to the understanding of PCV-3 epidemiology in Swiss pigs, emphasizing the need for continued surveillance and further research on its clinical implications and interaction with host factors. Our study underscores the utility and limitations of molecular techniques in confirming PCV-3 infections.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Diagnostic ; Histopathology ; In situ hybridization (ISH) ; PCV-3 systemic disease (PCV-3-SD) ; Porcine circovirus 3 (PCV-3) ; Real-time quantitative polymerase chain reaction (qPCR) ; Tissue microarray (TMA) ; Vascular lesions ; SDG 3 - Good Health and Well-being
Published in:	Viruses, Vol. 16 Núm. 9 (2024) , p. 1431, ISSN 1999-4915

DOI: 10.3390/v16091431
PMID: 39339907

10 p, 7.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles