Calorie restriction and rapamycin distinctly mitigate aging-associated protein phosphorylation changes in mouse muscles
Ataman, Meric (Swiss Institute of Bioinformatics)
Mittal, Nitish (University of Basel)
Tintignac, Lionel (University of Basel)
Schmidt, Alexander (University of Basel)
Ham, Daniel J. (University of Basel)
González, Asier (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ruegg, Markus A. (University of Basel)
Zavolan, Mihaela (Swiss Institute of Bioinformatics)
Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí"

Date:	2024
Abstract:	Calorie restriction (CR) and treatment with rapamycin (RM), an inhibitor of the mTORC1 growth-promoting signaling pathway, are known to slow aging and promote health from worms to humans. At the transcriptome and proteome levels, long-term CR and RM treatments have partially overlapping effects, while their impact on protein phosphorylation within cellular signaling pathways have not been compared. Here we measured the phosphoproteomes of soleus, tibialis anterior, triceps brachii and gastrocnemius muscles from adult (10 months) and 30-month-old (aged) mice receiving either a control, a calorie restricted or an RM containing diet from 15 months of age. We reproducibly detected and extensively analyzed a total of 6960 phosphosites, 1415 of which are not represented in standard repositories. We reveal the effect of these interventions on known mTORC1 pathway substrates, with CR displaying greater between-muscle variation than RM. Overall, CR and RM have largely consistent, but quantitatively distinct long-term effects on the phosphoproteome, mitigating age-related changes to different degrees. Our data expands the catalog of protein phosphorylation sites in the mouse, providing important information regarding their tissue-specificity, and revealing the impact of long-term nutrient-sensing pathway inhibition on mouse skeletal muscle.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cell signalling ; Data processing
Published in:	Communications Biology, Vol. 7 (August 2024) , art. 974, ISSN 2399-3642

DOI: 10.1038/s42003-024-06679-4
PMID: 39127848

12 p, 1.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles