Impact of Overnight Storage of Human Atrial Myocytes on Intracellular Calcium Homeostasis and Electrophysiological Utility
Aceituno, Cristina (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Revuelta, D. (Partner Site Hamburg/Kiel/Lübeck)
Jiménez-Sábado, Verónica (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ginel, Antonino (Institut de Recerca Sant Pau)
Molina, C.E. (Partner Site Hamburg/Kiel/Lübeck)
Madsen, Leif Hove (Institut de Recerca Sant Pau)
Universitat Autònoma de Barcelona

Date:	2024
Abstract:	Human atrial myocytes afford an attractive experimental model to investigate mechanisms underlying electrophysiological alterations in cardiovascular disease. However, this model presents limitations, such as the availability of human atrial tissue and a variable yield of myocytes isolation. Therefore, we aimed to determine whether overnight storage can increase the time window where the electrophysiological properties of human atrial myocytes can be determined. To address this issue, human atrial myocytes isolated from patients undergoing cardiac surgery were used for patch-clamp experiments on the day of cell isolation (Day 1) and the following day (Day 2). The shape of the current-voltage (I-V) relationship for the calcium current (I) depended on the access resistance and the cell capacitance, with large cells (.
Grants:	Agencia Estatal de Investigación PID2020-116927RB-C21 Agencia Estatal de Investigación PID2023-152610OB-C21
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Beat-to-beat response ; Calcium currents ; Calcium homeostasis ; Human atrial myocyte ; Patch-clamp technique ; Sarcoplasmic reticulum
Published in:	Biomolecules, Vol. 14 Núm. 11 (november 2024) , p. 1415, ISSN 2218-273X

DOI: 10.3390/biom14111415
PMID: 39595591

11 p, 2.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles