No evidence of mutations associated with anticoagulant resistance in gene Vkorc1 in brown and black rats from Barcelona
Ruiz-López, María José (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Franco Cirera, Sandra (Agència de Salut Pública de Barcelona)
Martínez de la Puente, Josué (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Ferraguti, Martina (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Miccolis, Emanuele (Third Sector International (3Si))
Petit, Robert (Agència de Salut Pública de Barcelona)
Barahona Quintana, Laura (Institut de Recerca Sant Pau)
Figuerola Borras, Jordi (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Montalvo Porro, Tomas (Institut de Recerca Sant Pau)
Universitat Autònoma de Barcelona

Fecha:	2024
Resumen:	Synanthropic rodents such as the brown rat (Rattus norvegicus) and black rat (Rattus rattus) are a source of disturbance in urban areas and the focus of control programs. Control measures often rely on anticoagulant rodenticides, but their broad use is compromised by the emergence of resistance. Here we studied the prevalence of anticoagulant resistance genotypes in the Vkorc1 gene in rats in the metropolitan area of Barcelona. In this area, part of the management practices to control brown rats include anticoagulant rodenticide use, but rodenticides with different active ingredients are used in rotation. Brown rats were sampled from the sewage system during two periods: from December 2016 to November 2017 when difenacoum and brodifacoum were used, and from August 2021 to July 2022 when bromadiolone was used. Because black rats have just recently been detected in Barcelona, we only studied them during the latter sampling period, with samples obtained from a control action carried out in a green urban area. Exon 3 of the Vkorc1 gene was characterized in both species, while exon 1 was additionally analyzed in black rats. Synonymous mutations, not resulting in amino-acid changes, were found in both exons, indicating no evidence of anticoagulant resistance in the rats of Barcelona. This finding indicates that the current rodent management plan in Barcelona, which involves anticoagulant rotation for brown rats and the use of life capture traps in specific situations for black rats, has helped to prevent the emergence of resistance to anticoagulant rodenticides in rats in Barcelona. Future actions should aim to diversify the control measures included in the rodent management plan.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Commensal rodents ; Pest control ; Rattus norvegicus ; Rattus rattus ; Urban ; Vkorc1
Publicado en:	Science of the total environment, Vol. 954 (january 2024) , p. 176321, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2024.176321
PMID: 39341232

7 p, 3.3 MB

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