Isolation of Staphylococcus pseudintermedius in Immunocompromised Patients from a Single Center in Spain : A Zoonotic Pathogen from Companion Animals
Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Verdejo, Miguel Ángel (Hospital Clínic i Provincial de Barcelona)
Horvath, Laura (Hospital Clínic i Provincial de Barcelona)
Vergara, Andrea (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Vila Estapé, Jordi (Centro de Investigación Biomédica en Red de Enfermedades Infecciosas)
Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Morata, Laura (Universitat de Barcelona. Departament de Fonaments Clínics)
Espasa, Mateu (Universitat de Barcelona. Departament de Fonaments Clínics)
Casals-Pascual, Climent (Centro de Investigación Biomédica en Red de Enfermedades Infecciosas)
Soriano Viladomiu, Alex (Centro de Investigación Biomédica en Red de Enfermedades Infecciosas)
Pitart, Cristina (Universitat de Barcelona. Departament de Fonaments Clínics)

Date:	2024
Abstract:	Staphylococcus pseudintermedius, a commensal opportunistic bacterium predominantly residing in the skin of companion animals, particularly dogs, has the potential to induce skin and soft tissue infections in pets, and zoonotic infections, including catheter-related complications. This study documents four cases of S. pseudintermedius infection or colonization in patients who had close contact with dogs or cats. Identification of the bacterial species was performed using MALDI-TOF mass spectrometry, and antibiotic susceptibility was determined using microdilution assay. DNA was sequenced using Nanopore technology followed by in silico analysis. Three isolates were multidrug resistant, including resistance to methicillin, with one belonging to the prevalent European lineage ST551, and the other two were attributed to a novel multilocus sequence type, ST2672. The remaining isolate was attributed to the novel multilocus sequence type ST2673 and was methicillin susceptible. All four isolates exhibited an array of virulence factors that contributed to colonization, damage to host immune cells, and biofilm formation. All the ST551 isolates included in the comparative analysis displayed clonality within the European continent. The importance of describing zoonotic infections associated with S. pseudintermedius resides in the scarcity of available scientific literature, further accentuated by its heightened resistance profile and potential complications, particularly in the context of catheter-related infections.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Staphylococcus pseudintermedius ; ST551 ; MRSP ; MDR ; Zoonosis ; SSTI ; Whole-genome sequencing ; Catheter-related infection ; Nanopore ; One health
Published in:	Microorganisms, Vol. 12 (august 2024) , ISSN 2076-2607

DOI: 10.3390/microorganisms12081695
PMID: 39203536

15 p, 2.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles