Clonal dissemination of Acinetobacter radioresistens among Humboldt penguins (Spheniscus humboldti) inhabiting a barren northern Peruvian island
Roca, Ignasi (Centro de Investigación Biomédica en Red de Enfermedades Infecciosas)
Espinoza, Kathya (Universidad Científica del Sur)
Irigoin-Lovera, Cinthia (Universidad Científica del Sur)
Piquet, Maria (Hospital Clínic i Provincial de Barcelona)
Palomino-Kobayashi, Luciano A. (Universidad Científica del Sur)
Castillo, Angie K. (Universidad Científica del Sur)
Gonzales-DelCarpio, Diego D. (Universidad Científica del Sur)
Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Muñoz, Laura (Centro de Investigación Biomédica en Red de Enfermedades Infecciosas)
Ymaña, Barbara (Universidad Científica del Sur)
Oporto, Rosario (Universidad Científica del Sur)
Zavalaga, Carlos (Universidad Científica del Sur)
Pons, Maria J. (Universidad Científica del Sur)
Ruiz, Joaquim (Universidad Científica del Sur)

Date:	2024
Abstract:	Acinetobacter spp. are often isolated from natural sources, but knowledge about their presence in wild animals is fragmented and uncomplete. The present study aimed to characterize a series of Acinetobacter radioresistens isolated from Humboldt penguins (Spheniscus humboldti). Fifteen Humboldt penguins from an inhabited northern Peruvian island were sampled. Microorganisms were identified by MALDI-TOF MS. Antibiotic susceptibility to 12 antimicrobial agents was established, and clonal relationships were determined. A representative isolate was selected for whole genome sequencing (WGS). A. radioresistens were isolated from the feces of 12 (80%) Humboldt penguins, being susceptible to all the antimicrobial agents tested, except eight cefotaxime-intermediate isolates. All A. radioresistens were clonally related. WGS showed that the isolate belonged to ST1972, the presence of two chromosomal encoded carbapenemases (bla and a putative subclass B3 metallo-β-lactamase), and a series of point mutations in antibiotic-resistance related chromosomal genes, which were considered as polymorphisms. In addition, a few virulence factors, including a capsule-encoding operon, superoxide dismutases, catalases, phospholipases and a siderophore receptor were identified. The present results suggest that A. radioresistens may be a common member of the gut microbiota of Humboldt penguins, but further studies in other geographical areas are needed to establish this finding.
Grants:	Ministerio de Ciencia, Innovación y Universidades CEX2018-000806-S Ministerio de Ciencia e Innovación PID2021-127402OB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-0809
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Acinetobacter spp ; One health ; South America ; Antibiotic susceptibility ; OXA-23
Published in:	European Journal of Microbiology & Immunology, Vol. 14 (march 2024) , p. 210-218, ISSN 2062-8633

DOI: 10.1556/1886.2023.00066
PMID: 38483509

9 p, 1.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles