Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
Rey-Velasco, Xavier (Institut de Ciències del Mar)
Lucena, Teresa (Universitat de València)
Belda, Ana (Universitat de València)
Gasol, Josep M. (Institut de Ciències del Mar)
Sánchez Martínez, M. Olga (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Arahal, David R. (Universitat de València)
Pujalte, María J. (Universitat de València)

Data:	2024
Resum:	Prokaryotes dominate global oceans and shape biogeochemical cycles, yet most taxa remain uncultured and uncharacterized as of today. Here we present the characterization of 26 novel marine bacterial strains from a large isolate collection obtained from Blanes Bay (NW Mediterranean) microcosm experiments made in the four seasons. Morphological, cultural, biochemical, physiological, nutritional, genomic, and phylogenomic analyses were used to characterize and phylogenetically place the novel isolates. The strains represent 23 novel bacterial species and six novel genera: three novel species pertaining to class Alphaproteobacteria (families Rhodobacteraceae and Sphingomonadaceae), six novel species and three new genera from class Gammaproteobacteria (families Algiphilaceae, Salinispheraceae, and Alteromonadaceae), 13 novel species and three novel genera from class Bacteroidia (family Flavobacteriaceae), and one new species from class Rhodothermia (family Rubricoccaceae). The bacteria described here have potentially relevant roles in the cycles of carbon (e. g. , carbon fixation or energy production via proteorhodopsin), nitrogen (e. g. , denitrification or use of urea), sulfur (oxidation of sulfur compounds), phosphorus (acquisition and use of different forms of phosphorus and remodeling of membrane phospholipids), and hydrogen (oxidation of hydrogen to obtain energy). We mapped the genomes of the presented strains to the Tara Oceans metagenomes to reveal that these strains were globally distributed, with those of the family Flavobacteriaceae being the most widespread and abundant, while Rhodothermia being the rarest and most localized. While molecular-only approaches are also important, our study stresses the importance of culturing as a powerful tool to further understand the functioning of marine bacterial communities.
Ajuts:	Agencia Estatal de Investigación RTI2018-101025-B-I00 Agencia Estatal de Investigación PID2021-125469NB-C31 Agencia Estatal de Investigación PID2021-125469NB-C32 Ministerio de Ciencia e Innovación CEX2019-000928-S
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Taxonomy ; Genomics ; Phylogenomics ; Isolates ; Marine bacteria ; Biogeochemistry ; Biogeography
Publicat a:	Frontiers in microbiology, Vol. 15 (May 2024) , art. 1407904, ISSN 1664-302X

DOI: 10.3389/fmicb.2024.1407904
PMID: 38863746

30 p, 9.3 MB

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