Host genetic variation and specialized metabolites from wheat leaves enriches for phyllosphere Pseudomonas spp. with enriched antibiotic resistomes
Xiang, Qian (Chinese Academy of Sciences)
Lin, Da (Chinese Academy of Sciences)
Yang, Zai-Jun (China West Normal University)
Han, Rui-Xia (Chinese Academy of Sciences)
Zhang, Tian-Lun (Chinese Academy of Sciences)
Chen, Qing-Lin (Chinese Academy of Sciences)
Zhu, Dong (Chinese Academy of Sciences)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Zhu, Yong-Guan (Chinese Academy of Sciences)

Data:	2024
Resum:	Antibiotic resistance in plant-associated microbiomes poses significant risks for agricultural ecosystems and human health. Although accumulating evidence suggests a role for plant genotypes in shaping their microbiome, almost nothing is known about how the changes of plant genetic information affect the co-evolved plant microbiome carrying antibiotic resistance genes (ARGs). Here, we selected 16 wheat cultivars and experimentally explored the impact of host genetic variation on phyllosphere microbiome, ARGs, and metabolites. Our results demonstrated that host genetic variation significantly influenced the phyllosphere resistomes. Wheat genotypes exhibiting high phyllosphere ARGs were linked to elevated Pseudomonas populations, along with increased abundances of Pseudomonas aeruginosa biofilm formation genes. Further analysis of 350 Pseudomonas spp. genomes from diverse habitats at a global scale revealed that nearly all strains possess multiple ARGs, virulence factor genes (VFGs), and mobile genetic elements (MGEs) on their genomes, albeit with lower nucleotide diversity compared to other species. These findings suggested that the proliferation of Pseudomonas spp. in the phyllosphere significantly contributed to antibiotic resistance. We further observed direct links between the upregulated leaf metabolite DIMBOA-Glc, Pseudomonas spp. , and enrichment of phyllosphere ARGs, which were corroborated by microcosm experiments demonstrating that DIMBOA-Glc significantly enhanced the relative abundance of Pseudomonas spp. Overall, alterations in leaf metabolites resulting from genetic variation throughout plant evolution may drive the development of highly specialized microbial communities capable of enriching phyllosphere ARGs. This study enhances our understanding of how plants actively shape microbial communities and clarifies the impact of host genetic variation on the plant resistomes.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Antibiotic resistance genes ; Plant-microbe co-evolution ; Pseudomonas spp ; Metabolites ; Plant genetic variation
Publicat a:	The ISME Journal, Vol. 18, Issue 1 (January 2024) , art. wrae144, ISSN 1751-7370

DOI: 10.1093/ismejo/wrae144
PMID: 39073904

12 p, 3.6 MB

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