The trichome pattern diversity of Cardamine shares genetic mechanisms with Arabidopsis but differs in environmental drivers
Fuster-Pons, Alberto (Centro Nacional de Biotecnología)
Murillo-Sánchez, Alba (Centro Nacional de Biotecnología)
Méndez-Vigo, Belén (Centro Nacional de Biotecnología)
Marcer, Arnald (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Pieper, Bjorn (Max Planck Institute for Plant Breeding Research)
Torres-Pérez, Rafael (Centro Nacional de Biotecnología)
Oliveros, Juan Carlos (Centro Nacional de Biotecnología)
Tsiantis, Miltos (Max Planck Institute for Plant Breeding Research)
Picó, Xavier (Estación Biológica de Doñana)
Alonso-Blanco, Carlos (Centro Nacional de Biotecnología)

Data:	2024
Resum:	Natural variation in trichome pattern (amount and distribution) is prominent among populations of many angiosperms. However, the degree of parallelism in the genetic mechanisms underlying this diversity and its environmental drivers in different species remain unclear. To address these questions, we analyzed the genomic and environmental bases of leaf trichome pattern diversity in Cardamine hirsuta, a relative of Arabidopsis (Arabidopsis thaliana). We characterized 123 wild accessions for their genomic diversity, leaf trichome patterns at different temperatures, and environmental adjustments. Nucleotide diversities and biogeographical distribution models identified two major genetic lineages with distinct demographic and adaptive histories. Additionally, C. hirsuta showed substantial variation in trichome pattern and plasticity to temperature. Trichome amount in C. hirsuta correlated positively with spring precipitation but negatively with temperature, which is opposite to climatic patterns in A. thaliana. Contrastingly, genetic analysis of C. hirsuta glabrous accessions indicated that, like for A. thaliana, glabrousness is caused by null mutations in ChGLABRA1 (ChGL1). Phenotypic genome-wide association studies (GWAS) further identified a ChGL1 haplogroup associated with low trichome density and ChGL1 expression. Therefore, a ChGL1 series of null and partial loss-of-function alleles accounts for the parallel evolution of leaf trichome pattern in C. hirsuta and A. thaliana. Finally, GWAS also detected other candidate genes (e. g. ChETC3, ChCLE17) that might affect trichome pattern. Accordingly, the evolution of this trait in C. hirsuta and A. thaliana shows partially conserved genetic mechanisms but is likely involved in adaptation to different environments.
Ajuts:	Agencia Estatal de Investigación BES-2017-080063 Ministerio de Ciencia e Innovación PRE2020-094891 Agencia Estatal de Investigación PID2022-136893NB-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Plant physiology, Vol. 196, Issue 4 (December 2024) , p. 2730-2748, ISSN 1532-2548

DOI: 10.1093/plphys/kiae213
PMID: 38606947

19 p, 1.8 MB

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