Production of MLM Type Structured Lipids From Grapeseed Oil Catalyzed by Non-Commercial Lipases
Costa, Carolina M. (Universidade de Lisboa. Instituto Superior de Agronomia)
Osório, Natália M. (Instituto Politécnico de Setúbal)
Canet Morral, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Rivera, Ivanna (Centro de Investigación y Asistencia en Tecnologia y Diseño del Estado de Jalisco)
Sandoval, Georgina (Centro de Investigación y Asistencia en Tecnologia y Diseño del Estado de Jalisco)
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Ferreira-Dias, Suzana (Universidade de Lisboa. Instituto Superior de Agronomia)

Data:	2018
Resum:	Low calorie triacylglycerols (TAG) presenting medium-chain fatty acids (M) at positions sn-1,3 and long-chain fatty acids (L) at position sn-2 are known as MLM. This study aims at the production of MLM by acidolysis of grapeseed oil with medium-chain caprylic (C8:0) or capric (C10:0) acids. Grapeseed oil is used as source of long-chain polyunsaturated fatty acids (FAs), especially linoleic acid, at sn-2 position in TAG. Reactions are performed in batch, in solvent-free systems, during 48 h. Novel non-commercial sn-1,3 regioselective lipases are used as alternative to high-cost commercial biocatalysts, namely, the heterologous lipase from Rhizopus oryzae (rROL) immobilized in Amberlite™ IRA 96 and Carica papaya lipase (CPL) self-immobilized in papaya latex. The highest productions of new TAG are achieved at 40 °C, molar ratio TAG:M of 1:2, after 48 h, with both biocatalysts, with yields varying between 38 and 69%. rROL immobilized in Amberlite IRA 96 shows a preference toward caprylic acid while CPL shows no preference toward caprylic or capric acid. First-order deactivation kinetics is observed for both biocatalysts. Half-lives at 40 °C are 166 and 118 h for rROL and 96 and 81 h for CPL, in the acidolysis of grapeseed oil with C8:0 or C10:0, respectively. Practical Applications: Grapeseeds of Vitis vinifera L. are a by-product of the wine industry. Using grapeseed oil to produce added-value functional oils rich in linoleic acid (essential fatty acid) may be a way of improving the revenues of the enological and oil sectors. Both lipases, and mainly Carica papaya lipase self-immobilized in papaya latex, due to its low-cost production and easy preparation, are promising non-commercial biocatalysts for the synthesis of MLM in solvent-free media. The use of a solvent-free system, in addition of being a green option, is also preferred for economic reasons, avoiding the costs with solvent and solvent recovery. Also, the use of the stoichiometric molar ratio TAG:M of 1:2 will 1) decrease costs related with the recovery and reutilization of medium chain fatty acids in excess, product recovery and purification, and 2) avoid biocatalyst deactivation by high amounts of free fatty acids in reaction media. Both Carica papaya lipase (CPL) self-immobilized in papaya latex and rROL in Amberlite IRA 96 catalyze the synthesis of MLM from grapeseed oil in solvent-free medium. The highest productions of new TAG are achieved at 40 °C, molar ratio TAG:M of 1:2, after 48 h, with both biocatalysts, with yields varying between 38 and 69%. rROL immobilized in Amberlite IRA96 shows a preference toward caprylic acid while CPL shows no preference toward caprylic or capric acid.
Ajuts:	Ministerio de Economía y Competitividad CTQ2013-42391-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-452
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Llengua:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Matèria:	Carica papaya lipase ; Rhizopus oryzae lipase ; Grapeseed oil ; Low calorie lipids ; Structured lipids
Publicat a:	European Journal of Lipid Science and Technology, Vol. 120, Issue 1 (January 2018) , art. 1700320, ISSN 1438-9312

DOI: 10.1002/ejlt.201700320

Preprint 58 p, 1.9 MB

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