Gene expression and TNF-alpha secretion profile in rainbow trout macrophages following exposures to copper and bacterial lipopolysaccharide
Teles, Mariana (Universitat Autònoma de Barcelona. Departament de Biologia Cel.lular, de Fisiologia i d'Immunologia)
Mackenzie, Simon (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Boltana, Sebastian (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Callol Junyer, Agnès (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel.lular, de Fisiologia i d'Immunologia)

Date:	2011
Abstract:	Fish macrophage function can be altered after exposure to pathogens as well as to xenobiotics. Considering that wild and farmed fish can be exposed in their habitats simultaneously to different types of stressors, including chemical contaminants (e. g. heavy metals) and pathogens (e. g. bacteria), it is fundamental to study their impact either isolated or in combination. Therefore, the present study aimed to evaluate the effects of copper and bacterial lipopolysaccharide (LPS), alone and in combination, on the transcription of target genes related with immune system, respiratory burst activity and cell death, using rainbow trout macrophages as in vitro model. A cell viability experiment was performed to determine the sub-lethal concentrations of copper for rainbow trout macrophages and the LC50-24 h was estimated at 60 μM. The expression of proinflammatory cytokines, such as interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor-α (TNFα) increased after copper and copper plus LPS exposure. Copper and LPS interact positively inducing an increase in cytokine expression, which may be indicative of an increased inflammatory response. However, the increase in TNFα mRNA expression induced by 50 μM copper was not accompanied by protein secretion indicating that mRNA abundance does not always reflect the level of protein and that the translation of the TNFα mRNA is somehow inhibited. Serum amyloid A (SAA) and trout C-polysaccharide binding protein (TCPBP) mRNA expression also increased after copper, LPS or LPS plus copper exposure, indicating a role of acute phase proteins in the local response to inflammation. NADPH oxidase and glutathione peroxidase gene expression increased in macrophages after 24 h exposure to copper, LPS or LPS plus copper. The results from the present study improve the understanding of mechanisms involved in copper toxicity, as well as the interaction with a simulated-inflammatory process.
Grants:	Ministerio de Educación y Ciencia AGL2006-03621 Ministerio de Ciencia e Innovación BFU2009-07354
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Copper ; Gene expression ; Lipopolysaccharide ; Macrophages ; Rainbow trout
Published in:	Fish & shellfish immunology, Vol. 30, Num. 1 (January 2011) , p. 340-346, ISSN 1095-9947

DOI: 10.1016/j.fsi.2010.11.006

Postprint 8 p, 554.3 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
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