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Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates
Seras Franzoso, Joaquin (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Peebo, Karl (Competence Centre of Food And Fermentation Technologies)
Garcia Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rinas, Ursula (Universität Hannover. Institut für Technische Chemie)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date: 2014
Abstract: Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37 °C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25 °C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.
Note: Altres ajuts: We are indebted CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN, Spain) for funding our research on inclusion bodies.
Note: Número d'acord de subvenció MINECO/BFU2010-17450
Note: Número d'acord de subvenció AGAUR/2019/SGR-0108
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: Drug delivery ; Inclusion bodies ; Nanomedicine ; Protein release ; Surface functionalization
Published in: Acta biomaterialia, Vol. 10 issue 3 (March 2014) , p. 1354-1359, ISSN 1742-7061

DOI: 10.1016/j.actbio.2013.12.021


Post-print
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Articles > Research articles
Articles > Published articles

 Record created 2015-06-18, last modified 2018-10-28



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