Intracellular targeting of CD44+ cells with self-assembling, protein only nanoparticles
Pesarrodona Roches, Mireia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gener, Petra (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Tatkiewicz, Witold (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Abasolo, Ibane (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Ratera Bastardas, Imma (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Veciana i Miró, Jaume (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Schwartz, Simo (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Date:	2014
Abstract:	CD44 is a multifunctional cell surface protein involved in proliferation and differentiation, angiogenesis and signaling. The expression of CD44 is up-regulated in several types of human tumors and particularly in cancer stem cells, representing an appealing target for drug delivery in the treatment of cancer. We have explored here several protein ligands of CD44 for the construction of self-assembling modular proteins designed to bind and internalize target cells. Among five tested ligands, two of them (A5G27 and FNI/II/V) drive the formation of protein-only, ring-shaped nanoparticles of about 14 nm that efficiently bind and penetrate CD44(+) cells by an endosomal route. The potential of these newly designed nanoparticles is evaluated regarding the need of biocompatible nanostructured materials for drug delivery in CD44-linked conditions.
Grants:	Instituto de Salud Carlos III FIS/PI12/00327 Instituto de Salud Carlos III FIS/PI12/01079 Ministerio de Economía y Competitividad BIO2013-41019-P Ministerio de Economía y Competitividad CTQ2010-19501 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-132
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Biomaterials ; CD44 ; Drug delivery ; Administració de medicaments ; Multifunctional protein ; Proteïnes multifuncionals ; Nanoparticle ; Nanopartícula ; Self-assembling ; Auto-ensamblatge
Published in:	International journal of pharmaceutics, Vol. 473, Issue 1-2 (Oct. 2014) , p. 286-295, ISSN 0378-5173

DOI: 10.1016/j.ijpharm.2014.07.016

Accepted article 31 p, 532.0 KB

Figures 4 p, 279.9 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles