Web of Science: 4 citations, Scopus: 4 citations, Google Scholar: citations
Redefining the PF06864 Pfam Family Based on Burkholderia pseudomallei PilO2Bp S-SAD Crystal Structure
Lassaux, Patricia (Università di Milano. Department of Biosciences)
Conchillo-Solé, Óscar (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Manjasetty, Babu A. (Grenoble Outstation. European Molecular Biology Laboratory)
Yero Corona, Daniel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Perletti, Lucia (Università di Milano. Department of Biosciences)
Belrhali, Hassan (Grenoble Outstation. European Molecular Biology Laboratory)
Daura i Ribera, Xavier (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Gourlay, Louise J. (Università di Milano. Department of Biosciences)
Bolognesi, Martino (Università di Milano. Department of Biosciences)

Date: 2014
Abstract: Type IV pili are surface-exposed filaments and bacterial virulence factors, represented by the Tfpa and Tfpb types, which assemble via specific machineries. The Tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. Here we focus on PilO2Bp, a protein component of the Tfpb R64 thin pilus variant assembly machinery from the pathogen Burkholderia pseudomallei. PilO2Bp belongs to the PF06864 Pfam family, for which an improved definition is presented based on newly derived Hidden Markov Model (HMM) profiles. The 3D structure of the N-terminal domain of PilO2Bp (N-PilO2Bp), here reported, is the first structural representative of the PF06864 family. N-PilO2Bp presents an actin-like ATPase fold that is shown to be present in BfpC, a different variant assembly protein; the new HMM profiles classify BfpC as a PF06864 member. Our results provide structural insight into the PF06864 family and on the Type IV pili assembly machinery.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Sequence alignment ; Multiple alignment calculation ; Hidden Markov models ; Crystal structure ; Phosphates ; Protein domains ; Protein structure comparison ; Pili and fimbriae
Published in: PloS one, Vol. 9 Issue 4 (April 2014) , p. e94981, ISSN 1932-6203

DOI: 10.1371/journal.pone.0094981
PMID: 24728008


10 p, 1.2 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2015-10-27, last modified 2020-04-25



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