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Analysis of porcine adipose tissue transcriptome reveals differences in de novo fatty acid synthesis in pigs with divergent muscle fatty acid composition
Corominas Galbany, Jordi (Centre de Recerca en Agrigenòmica)
Ramayo-Caldas, Yuliaxis (Centre de Recerca en Agrigenòmica)
Puig-Oliveras, Anna (Centre de Recerca en Agrigenòmica)
Estellé Fabrellas, Jordi (Institut national de la recherche agronomique (França). Génétique Animale et Biologie Intégrative)
Castelló, Anna (Centre de Recerca en Agrigenòmica)
Alves, Estefania (Institut national de la recherche agronomique (França). Génétique Animale et Biologie Intégrative)
Pena i Subirà, Ramona Natacha (Institut de Recerca i Tecnologia Agroalimentàries)
Ballester Devis, Maria (Centre de Recerca en Agrigenòmica)
Folch Albareda, Josep Maria (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date: 2013
Abstract: Background: In pigs, adipose tissue is one of the principal organs involved in the regulation of lipid metabolism. It is particularly involved in the overall fatty acid synthesis with consequences in other lipid-target organs such as muscles and the liver. With this in mind, we have used massive, parallel high-throughput sequencing technologies to characterize the porcine adipose tissue transcriptome architecture in six Iberian x Landrace crossbred pigs showing extreme phenotypes for intramuscular fatty acid composition (three per group). Results: High-throughput RNA sequencing was used to generate a whole characterization of adipose tissue (backfat) transcriptome. A total of 4,130 putative unannotated protein-coding sequences were identified in the 20% of reads which mapped in intergenic regions. Furthermore, 36% of the unmapped reads were represented by interspersed repeats, SINEs being the most abundant elements. Differential expression analyses identified 396 candidate genes among divergent animals for intramuscular fatty acid composition. Sixty-two percent of these genes (247/396) presented higher expression in the group of pigs with higher content of intramuscular SFA and MUFA, while the remaining 149 showed higher expression in the group with higher content of PUFA. Pathway analysis related these genes to biological functions and canonical pathways controlling lipid and fatty acid metabolisms. In concordance with the phenotypic classification of animals, the major metabolic pathway differentially modulated between groups was de novo lipogenesis, the group with more PUFA being the one that showed lower expression of lipogenic genes. Conclusions: These results will help in the identification of genetic variants at loci that affect fatty acid composition traits. The implications of these results range from the improvement of porcine meat quality traits to the application of the pig as an animal model of human metabolic diseases.
Note: Número d'acord de subvenció MICINN/AGL2011-29821-C02
Note: Número d'acord de subvenció MEC/BES-2009-081223
Note: Número d'acord de subvenció MEC/AP2008-01450
Note: Número d'acord de subvenció MICINN/CSD2007-00036
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: RNA-Seq ; Transcriptome ; Adipose tissue ; Pork ; De novo lipogenesis
Published in: BMC genomics, Vol. 14 N. 843 (December 2013) , p. 1-14, ISSN 1471-2164

DOI: 10.1186/1471-2164-14-843
PMID: 24289474


14 p, 1.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-03-08, last modified 2019-11-05



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