Nutrient supply affects the mRNA expression profile of the porcine skeletal muscle
Figueiredo Cardoso, Tainã (Centre de Recerca en Agrigenòmica)
Quintanilla, Raquel (Institut de Recerca i Tecnologia Agroalimentàries)
Tibau, Joan (Institut de Recerca i Tecnologia Agroalimentàries)
Gil, Marta (Institut de Recerca i Tecnologia Agroalimentàries)
Mármol-Sánchez, Emilio (Centre de Recerca en Agrigenòmica)
González-Rodríguez, Olga (Capes Foundation)
González Prendes, Rayner (Centre de Recerca en Agrigenòmica)
Amills i Eras, Marcel (Centre de Recerca en Agrigenòmica)

Date:	2017
Abstract:	Background: The genetic basis of muscle fat deposition in pigs is not well known. So far, we have only identified a limited number of genes involved in the absorption, transport, storage and catabolism of lipids. Such information is crucial to interpret, from a biological perspective, the results of genome-wide association analyses for intramuscular fat content and composition traits. Herewith, we have investigated how the ingestion of food changes gene expression in the gluteus medius muscle of Duroc pigs. Results: By comparing the muscle mRNA expression of fasted pigs (T0) with that of pigs sampled 5 h (T1) and 7 h (T2) after food intake, we have detected differential expression (DE) for 148 (T0-T1), 520 (T0-T2) and 135 (T1-T2) genes (q-value <0. 05 and a (FC) > of 1. 5). Many of these DE genes were transcription factors, suggesting that we have detected the coordinated response of the skeletal muscle to nutrient supply. We also found DE genes with a dual role in oxidative stress and angiogenesis (THBS1, THBS2 and TXNIP), two biological processes that are probably activated in the post-prandial state. Finally, we have identified several loci playing a key role in the modulation of circadian rhythms (ARNTL, PER1, PER2, BHLHE40, NR1D1, SIK1, CIART and CRY2), a result that indicates that the porcine muscle circadian clock is modulated by nutrition. Conclusion: We have shown that hundreds of genes change their expression in the porcine skeletal muscle in response to nutrient intake. Many of these loci do not have a known metabolic role, a result that suggests that our knowledge about the genetic basis of muscle energy homeostasis is still incomplete.
Grants:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad AGL2013-48742-C2-1-R Ministerio de Economía y Competitividad AGL2013-48742-C2-2-R Ministerio de Educación, Cultura y Deporte FPU15/01733 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1528
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Pig ; RNA-seq ; Oxidative stress ; Transcription factor ; Circadian rhythm ; Angiogenesis
Published in:	BMC genomics, Vol. 18 (Aug. 2017) , art. 603, ISSN 1471-2164

DOI: 10.1186/s12864-017-3986-x
PMID: 28797239

11 p, 2.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles