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Side effects of chaperone gene co-expression in recombinant protein production
Martínez Alonso, Mónica (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
García Fruitós, Elena (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona))
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rinas, Ursula (Leibniz Universität Hannover)
Villaverde, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date: 2010
Abstract: Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones along with foreign polypeptides is a common approach to increase the yield of properly folded, recombinant proteins in bacterial cell factories. However, unbalanced amounts of folding modulators handling folding-reluctant protein species might instead trigger undesired proteolytic activities, detrimental regarding recombinant protein stability, quality and yield. This minireview summarizes the most recent observations of chaperone-linked negative side effects, mostly focusing on DnaK and GroEL sets, when using these proteins as folding assistant agents. These events are discussed in the context of the complexity of the cell quality network and the consequent intricacy of the physiological responses triggered by protein misfolding.
Note: Número d'acord de subvenció MEC/BIO2007-61194
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: Microbial Cell Factories, Vol. 9, Issue 64 (September 2010) , ISSN 1475-2859

PMID: 20813055
DOI: 10.1186/1475-2859-9-64


6 p, 410.5 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2017-12-20, last modified 2019-01-23



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