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Intracellular CXCR4 + cell targeting with T22-empowered protein-only nanoparticles
Unzueta Elorza, Ugutz (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Céspedes, María Virtudes (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona))
Ferrer-Miralles, Neus (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Casanova, Isolda (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona))
Cedano, Juan (Laboratory of Immunology, Regional Norte, Universidad de la Republica, Salto, Uruguay)
Corchero, José Luis (CIBER en Bioingeniería, Biomateriales y Nanomedicina, Bellaterra, Barcelona)
Domingo-Espín, Joan (CIBER en Bioingeniería, Biomateriales y Nanomedicina, Bellaterra, Barcelona)
Villaverde, Antonio (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Mangues, Ramón (CIBER en Bioingeniería, Biomateriales y Nanomedicina, Bellaterra, Barcelona)
Vázquez, Esther (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia

Date: 2012
Abstract: Cell-targeting peptides or proteins are appealing tools in nanomedicine and innovative medicines because they increase the local drug concentration and reduce potential side effects. CXC chemokine receptor 4 (CXCR4) is a cell surface marker associated with several severe human pathologies, including colorectal cancer, for which intracellular targeting agents are currently missing. Four different peptides that bind CXCR4 were tested for their ability to internalize a green fluorescent protein-based reporter nanoparticle into CXCR4 + cells. Among them, only the 18 mer peptide T22, an engineered segment derivative of polyphemusin II from the horseshoe crab, efficiently penetrated target cells via a rapid, receptor-specific endosomal route. This resulted in accumulation of the reporter nanoparticle in a fully fluorescent and stable form in the perinuclear region of the target cells, without toxicity either in cell culture or in an in vivo model of metastatic colorectal cancer. Given the urgent demand for targeting agents in the research, diagnosis, and treatment of CXCR4-linked diseases, including colorectal cancer and human immunodeficiency virus infection, T22 appears to be a promising tag for the intracellular delivery of protein drugs, nanoparticles, and imaging agents.
Note: Número d'acord de subvenció MICINN/FIS/PS09/00165
Note: Número d'acord de subvenció MICINN/FIS/PS09/00965
Note: Número d'acord de subvenció MICINN/ACI2009-0919
Note: Número d'acord de subvenció AGAUR/2009/SGR-108
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Peptide tag ; CXCR4 ; Intracellular targeting ; Self-assembling ; Nanoparticles ; Colorectal cancer
Published in: International Journal of Nanomedicine, Vol. 7 (august 2012) , p. 4533-4544, ISSN 1178-2013

PMID: 22923991
DOI: 10.2147/IJN.S34450


12 p, 5.7 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2018-01-26, last modified 2019-01-25



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