Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives
Roca, Mercè (Fundació Centre de Recerca en Sanitat Animal)
Nofrarías, Miquel (Fundació Centre de Recerca en Sanitat Animal)
Majó i Masferrer, Natàlia (Universitat Autònoma de Barcelona. Departament de Sanitat i Anatomia Animals)
Pérez de Rozas, Ana María
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i Anatomia Animals)
Castillo, Marisol (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Martín Orúe, Susana M. (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Espinal, Anna (Universitat Autònoma de Barcelona. Servei d'Estadística Aplicada)
Pujols i Romeu, Joan (Fundació Centre de Recerca en Sanitat Animal)
Badiola Sáiz, Ignacio (Institut de Recerca i Tecnologia Agroalimentàries)

Date: 2014
Abstract: This study aimed to provide novel insights into the gastrointestinal microbial diversity from different gastrointestinal locations in weaning piglets using PCR-restriction fragment length polymorphism (PCR-RFLP). Additionally, the effect of different feed additives was analyzed. Thirty-two piglets were fed with four different diets: a control group and three enriched diets, with avilamycin, sodium butyrate, and a plant extract mixture. Digesta samples were collected from eight different gastrointestinal segments of each animal and the bacterial population was analysed by a PCR-RFLP technique that uses 16S rDNA gene sequences. Bacterial diversity was assessed by calculating the number of bands and the Shannon-Weaver index. Dendrograms were constructed to estimate the similarity of bacterial populations. A higher bacterial diversity was detected in large intestine compared to small intestine. Among diets, the most relevant microbial diversity differences were found between sodium butyrate and plant extract mixture. Proximal jejunum, ileum, and proximal colon were identified as those segments that could be representative of microbial diversity in pig gut. Results indicate that PCR-RFLP technique allowed detecting modifications on the gastrointestinal microbial ecology in pigs fed with different additives, such as increased biodiversity by sodium butyrate in feed.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: review ; ressenya ; publishedVersion ; article
Published in: BioMed Research International, Vol. 2014 (january 2014) , ISSN 2314-6141

PMID: 24575403

13 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Published articles
Articles > Reviews

 Record created 2018-01-29, last modified 2019-08-02

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