Coping with iron limitation : a metabolomic study of Synechocystis sp. PCC 6803
Rivas Ubach, Albert (Centre de Recerca Ecològica i Aplicacions Forestals)
Poret-Peterson, Amisha T. (Arizona State University. School of Earth and Space Exploration)
Peñuelas, Josep (Centre de Recerca Ecològica i Aplicacions Forestals)
Pérez-Trujillo, Miriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Legido-Quigley, Cristina (King’s College London. Institute of Pharmaceutical Science)
Oravec, Michal (Akademie věd České Republiky. Centrum výzkumu globální změny)
Urban, Otmar (Akademie věd České Republiky. Centrum výzkumu globální změny)
Elser, James J. (University of California. Crops Pathology and Genetics Research Unit)

Date: 2018
Abstract: Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA:DNA, C, N, and P, and higher ratios of protein:RNA, C:N, C:P, and N:P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C:P and N:P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn, and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. In addition, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular, and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.
Note: Número d'acord de subvenció EC/FP7/610028
Note: Número d'acord de subvenció MINECO/CGL2013-48074-P
Note: Número d'acord de subvenció AGAUR/2014/SGR-274
Rights: Tots els drets reservats
Language: Anglès
Document: article ; recerca ; acceptedVersion
Subject: Metabolomics ; Metallomics ; Iron limitation ; Cyanobacteria ; Ecological stoichiometry
Published in: Acta physiologiae plantarum, Vol. 40, art. 28 (Feb. 2018) , ISSN 0137-5881

DOI: 10.1007/s11738-018-2603-1

Available from: 2019-02-30

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals) > Imbalance-P
Articles > Research articles
Articles > Published articles

 Record created 2018-01-31, last modified 2018-02-02

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