Telomere attrition in heart failure : a flow-FISH longitudinal analysis of circulating monocytes
Teubel, Iris (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Elchinova, Elena Georgieva (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Roura, Santiago (Institut Germans Trias i Pujol)
Fernández, Marco A. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gálvez-Montón, Carolina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Moliner, Pedro (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
de Antonio Ferrer, Marta (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lupón, Josep (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Bayés-Genís, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)

Date:	2018
Abstract:	Cross-sectional investigations report shorter telomeres in patients with heart failure (HF); however, no studies describe telomere length (TL) trajectory and its relationship with HF progression. Here we aimed to investigate telomere shortening over time and its relationship to outcomes. Our study cohort included 101 ambulatory patients with HF. Blood samples were collected at baseline (n = 101) and at the 1-year follow-up (n = 54). Using flow-FISH analysis of circulating monocytes, we simultaneously measured three monocyte subsets-classical (CD14 ++ CD16 −), intermediate (CD14 ++ CD16 +), and nonclassical (CD14 + CD16 ++)-and their respective TLs based on FITC-labeled PNA probe hybridization. The primary endpoints were all-cause death and the composite of all-cause death or HF-related hospitalization, assessed at 2. 3 ± 0. 6 years. All statistical analyses were executed by using the SPSS 15. 0 software, and included Student's t test and ANOVA with post hoc Scheffe analysis, Pearson or Spearman rho correlation and univariate Cox regression when applicable. We found high correlations between TL values of different monocyte subsets: CD14 ++ CD16 + vs. CD14 ++ CD16 −, R = 0. 95, p < 0. 001; CD14 ++ CD16 + vs. CD14 + CD16 ++, R = 0. 90, p < 0. 001; and CD14 ++ CD16 − vs. CD14 + CD16 ++, R = 0. 89, p < 0. 001. Mean monocyte TL exhibited significant attrition from baseline to the 1-year follow-up (11. 1 ± 3. 3 vs. 8. 3 ± 2. 1, p < 0. 001). TL did not significantly differ between monocyte subsets at either sampling time-point (all p values > 0. 1). Cox regression analyses did not indicate that TL or ΔTL was associated with all-cause death or the composite endpoint. Overall, this longitudinal study demonstrated a ~ 22% reduction of TL in monocytes from ambulatory patients with HF within 1 year. TL and ΔTL were not related to outcomes over long-term follow-up. The online version of this article (10. 1186/s12967-018-1412-z) contains supplementary material, which is available to authorized users.
Grants:	Ministerio de Economía y Competitividad SAF2014-59892 Ministerio de Economía y Competitividad CB16/11/00403
Note:	Altres ajuts: Fundació La MARATÓ de TV3 (201502, 201516)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Heart failure ; Monocyte subsets ; Telomere attrition ; Telomere length
Published in:	Journal of translational medicine, Vol. 16 (february 2018) , ISSN 1479-5876

DOI: 10.1186/s12967-018-1412-z
PMID: 29463269

8 p, 964.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles