Monitoring t-cell responses in translational studies: optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-specific responses
Brinke, Anja ten (Sanquin Research (Amsterdam))
Marek-Trzonkowska,Natalia (Medical University of Gdansk. Department of Family Medicine)
Mansilla López, María José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Turksma, Annelies W. (Sanquin Research (Amsterdam))
Piekarska, Karolina (Medical University of Gdansk. Department of Family Medicine)
Iwaszkiewicz-Grzes, Dorota (Medical University of Gdansk. Department of Clinical Immunology and Transplantology)
Passerini, Laura (IRCCS San Raffaele Scientific Institute)
Locafaro, Grazia (IRCCS San Raffaele Scientific Institute)
Puñet Ortiz, Joan (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
van Ham, S. Marieke (Sanquin Research (Amsterdam))
Hernandez Fuentes, Maria P. (MRC Centre for Transplantation, King's College London)
Martínez Cáceres, Eva M. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gregori, Silvia (IRCCS San Raffaele Scientific Institute)
Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia

Fecha: 2017
Resumen: Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)- presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid organ and bone marrow transplantation. One of the critical issues to elucidate the mechanisms responsible for success or failure of these therapies and define the specificity of the therapy is the evaluation of the Ag-specific T-cell responses. Several efforts have been made to develop suitable and reproducible assays. Here, we focus on dye-based proliferation assays. We highlight with practical examples the fundamental issues to take into consideration for implementation of an effective and sensitive dye-based proliferation assay to monitor Ag-specific responses in patients. The most critical points were used to design a road map to set up and analyze the optimal assay to assess Ag-specific T-cell responses in patients undergoing different treatments. This is the first step to optimize monitoring of tolerance induction, allowing comparison of outcomes of different clinical studies. The road map can also be applied to other therapeutic interventions, not limited to tolerance induction therapies, in which Ag-specific T-cell responses are relevant such as vaccination approaches and cancer immunotherapy.
Nota: Altres ajuts: Polish Ministry of Science and Higher Education (grant no. IP2011 033771) and National Centre of Science, Poland (funding decision no. DEC-2011/01/D/NZ3/00262); National Centre for Research and Development (grants LIDER/160/L-6/14/NCBR/2015); grants G0801537/ID: 88245 and MR/J006742/1MR/J006742/1; grant from the Italian Telethon Foundation "Comitato Telethon Fondazione Onlus," Core grant TIGET TGT16G01 and the Italian Association for Cancer Research project IG 2013 N 14105
Nota: Número d'acord de subvenció EC/HEALTH-F5-2010-26068
Nota: Número d'acord de subvenció EC/ FP7-HEALTH-2012-INNOVATION-1/305147
Nota: Número d'acord de subvenció FIS/ PI14/01175
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès
Documento: article ; recerca ; publishedVersion
Materia: Tolerance ; Monitoring ; Proliferation ; Antigen-specific ; T cells ; Transplantation ; Autoimmune diseases ; Immune-therapies
Publicado en: Frontiers in immunology, Vol. 8 (december 2017) , p. 1-15, ISSN 1664-3224

15 p, 2.0 MB

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