Web of Science: 3 citations, Scopus: 2 citations, Google Scholar: citations,
Identification and characterization of new isoforms of human fas apoptotic inhibitory molecule (FAIM)
Coccia, Elena (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Calleja Yagüe, Isabel (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Planells Ferrer, Laura (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Sanuy, Blanca (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Sanz, Belen (Cell Division and Cancer Group. Spanish National Cancer Research Centre (CNIO))
López Soriano, Joaquin (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Moubarak, Rana S. (Department of Pathology. NYU Langone Medical Center)
Munell Casadesus, Francina (Vall d'Hebron Institut de Recerca(VHIR))
Barneda Zahonero, Bruna (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Comella i Carnicé, Joan Xavier (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)
Pérez García, María José (Universitat Autònoma de Barcelona. Institut de Neurociències. Departament de Bioquímica i Biologia Molecular)

Date: 2017
Abstract: Fas Apoptosis Inhibitory Molecule (FAIM) is an evolutionarily highly conserved death receptor antagonist, widely expressed and known to participate in physiological and pathological processes. Two FAIM transcript variants have been characterized to date, namely FAIM short (FAIM-S) and FAIM long (FAIM-L). FAIM-S is ubiquitously expressed and serves as an anti-apoptotic protein in the immune system. Furthermore, in neurons, this isoform promotes NGF-induced neurite outgrowth through NF-кB and ERK signaling. In contrast FAIM-L is found only in neurons, where it exerts anti-apoptotic activity against several stimuli. In addition to these two variants, in silico studies point to the existence of two additional isoforms, neither of which have been characterized to date. In this regard, here we confirm the presence of these two additional FAIM isoforms in human fetal brain, fetal and adult testes, and placenta tissues. We named them FAIM-S_2a and FAIM-L_2a since they have the same sequence as FAIM-S and FAIM-L, but include exon 2a. PCR and western blot revealed that FAIM-S_2a shows ubiquitous expression in all the tissues and cellular models tested, while FAIM-L_2a is expressed exclusively in tissues of the nervous system. In addition, we found that, when overexpressed in non-neuronal cells, the splicing factor nSR100 induces the expression of the neuronal isoforms, thus identifying it as responsible for the generation of FAIM-L and FAIM-L_2a. Functionally, FAIM-S_2a and FAIM-L_2a increased neurite outgrowth in response to NGF stimulation in a neuronal model. This observation thus, supports the notion that these two isoforms are involved in neuronal differentiation. Furthermore, subcellular fractionation experiments revealed that, in contrast to FAIM-S and FAIM-L, FAIM-S_2a and FAIM-L_2a are able to localize to the nucleus, where they may have additional functions. In summary, here we report on two novel FAIM isoforms that may have relevant roles in the physiology and pathology of the nervous system.
Note: Altres ajuts: La Marató de TV3 (201414-30); Fellowship BES-2014-069550
Note: Número d'acord de subvenció MINECO/SAF2013-47989-R
Note: Número d'acord de subvenció MINECO/SAF2016-80236-R
Note: Número d'acord de subvenció MINECO/CIBERNED CB06/05/1104
Note: Número d'acord de subvenció MINECO/PIE13/00027
Note: Número d'acord de subvenció AGAUR/2014SGR1609
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Alternative Splicing ; Animals ; Apoptosis Regulatory Proteins ; Cell Line ; Exons ; Humans ; Nucleic Acid Conformation ; PC12 Cells ; Protein Isoforms ; Protein Stability ; Rats ; Real-Time Polymerase Chain Reaction ; RNA, Messenger ; Thermodynamics
Published in: PLoS ONE, Vol. 12 Núm. 10 (october 2017) , p. e0185327, ISSN 1932-6203

DOI: 10.1371/journal.pone.0185327
PMID: 28981531


21 p, 6.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles

 Record created 2019-01-21, last modified 2019-07-21



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