The effector AWR5 from the plant pathogen Ralstonia solanacearum is an inhibitor of the TOR signalling pathway
Popa, Crina (Centre de Recerca en Agrigenòmica)
Liang, Li (Centre de Recerca en Agrigenòmica)
Gil, Sergio (Universitat de Barcelona. Departament de Genètica)
Tatjer, Laura (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Hashii, Keisuke (Kagawa University. Laboratory of Applied Molecular and Cell Biology)
Tabuchi, Mitsuaki (Kagawa University. Laboratory of Applied Molecular and Cell Biology)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Valls, Marc (Centre de Recerca en Agrigenòmica)

Date:	2016
Abstract:	Bacterial pathogens possess complex type III effector (T3E) repertoires that are translocated inside the host cells to cause disease. However, only a minor proportion of these effectors have been assigned a function. Here, we show that the T3E AWR5 from the phytopathogen Ralstonia solanacearum is an inhibitor of TOR, a central regulator in eukaryotes that controls the switch between cell growth and stress responses in response to nutrient availability. Heterologous expression of AWR5 in yeast caused growth inhibition and autophagy induction coupled to massive transcriptomic changes, unmistakably reminiscent of TOR inhibition by rapamycin or nitrogen starvation. Detailed genetic analysis of these phenotypes in yeast, including suppression of AWR5-induced toxicity by mutation of CDC55 and TPD3, encoding regulatory subunits of the PP2A phosphatase, indicated that AWR5 might exert its function by directly or indirectly inhibiting the TOR pathway upstream PP2A. We present evidence in planta that this T3E caused a decrease in TOR-regulated plant nitrate reductase activity and also that normal levels of TOR and the Cdc55 homologues in plants are required for R. solanacearum virulence. Our results suggest that the TOR pathway is a bona fide T3E target and further prove that yeast is a useful platform for T3E function characterisation.
Grants:	Ministerio de Economía y Competitividad AGL2013-46898-R Ministerio de Ciencia e Innovación AGL2010-21870 Ministerio de Economía y Competitividad BFU2011-30197-C3-01 Ministerio de Economía y Competitividad BFU2014-54591-C2-1-P Agència de Gestió d'Ajuts Universitaris i de Recerca BP_B 00030 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014SGR4
Note:	Aquest article té una correcció a 10.1038/s41598-020-78190-9
Note:	Altres ajuts: EU-Marie Curie Actions (PCDMC-321738 and PIIF-331392)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Ralstonia solanacearum
Published in:	Scientific reports, Vol. 6 (2016) , art. 27058, ISSN 2045-2322

Correcció de l'article: https://ddd.uab.cat/record/253130
DOI: 10.1038/srep27058
PMID: 27257085

14 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles