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Cerca | Lliura | Ajuda | Servei de Biblioteques | Sobre el DDD | Català English Español |
Pàgina inicial > Articles > Articles publicats > ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana |
Data: | 2008 |
Resum: | Xyloglucan endotransglucosylase/hydrolases (XTHs; EC 2. 4. 1. 207 and/or EC 3. 2. 1. 151) are enzymes involved in the modification of cell wall structure by cleaving and, often, also re-joining xyloglucan molecules in primary plant cell walls. Using a pool of antibodies raised against an enriched cell wall protein fraction, a new XTH cDNA in maize, ZmXTH1, has been isolated from a cDNA expression library obtained from the elongation zone of the maize root. The predicted protein has a putative N-terminal signal peptide and possesses the typical domains of this enzyme family, such as a catalytic domain that is homologous to that of Bacillus macerans β-glucanase, a putative N-glycosylation motif, and four cysteine residues in the central and C terminal regions of the ZmXTH1 protein. Phylogenetic analysis of ZmXTH1 reveals that it belongs to subgroup 4, so far only reported from Poaceae monocot species. ZmXTH1 has been expressed in Pichia pastoris (a methylotrophic yeast) and the recombinant enzyme showed xyloglucan endotransglucosylase but not xyloglucan endohydrolase activity, representing the first enzyme belonging to subgroup 4 characterized in maize so far. Expression data indicate that ZmXTH1 is expressed in elongating tissues, modulated by culture conditions, and induced by gibberellins. Transient expression assays in onion cells reveal that ZmXTH1 is directed to the cell wall, although weakly bound. Finally, Arabidopsis thaliana plants expressing ZmXTH1 show slightly increased xyloglucan endohydrolase activity and alterations in the cell wall structure and composition. |
Ajuts: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2003/FI00090 Ministerio de Ciencia y Tecnología BIO2001-1140 Agència de Gestió d'Ajuts Universitaris i de Recerca 2005/BE00104 Agència de Gestió d'Ajuts Universitaris i de Recerca 2006/BE00668 Agència de Gestió d'Ajuts Universitaris i de Recerca 2003/PIV-A-00033 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Cell elongation ; Cell wall ; Plant transformation ; XEH ; XET ; XTH ; Zea mays |
Publicat a: | Journal of experimental botany, Vol. 59, Issue 4 (March 2008) , p. 875-889, ISSN 1460-2431 |
15 p, 1.7 MB |