Leaves of isoprene-emitting tobacco plants maintain PSII stability at high temperatures
Pollastri, Susanna (Institute for Sustainable Plant Protection (Florència, Itàlia))
Jorba, Ignasi (Institut de Bioenginyeria de Catalunya)
Hawkins, Timothy J. (Durham University. Department of Biosciences)
Llusia, Joan (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Michelozzi, Marco (Istituto di Bioscienze e Biorisorse (Florència, Itàlia))
Navajas, Daniel (Institut de Bioenginyeria de Catalunya)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Hussey, Patrick J. (Durham University. Department of Biosciences)
Knight, Marc R. (Durham University. Department of Biosciences)
Loreto, Francesco (Università degli Studi di Napoli Federico II. Dipartimenti di Biologia)

Data:	2019
Resum:	At high temperatures, isoprene-emitting plants display a higher photosynthetic rate and a lower nonphotochemical quenching (NPQ) compared with nonemitting plants. The mechanism of this phenomenon, which may be very important under current climate warming, is still elusive. - NPQ was dissected into its components, and chlorophyll fluorescence lifetime imaging microscopy (FLIM) was used to analyse the dynamics of excited chlorophyll relaxation in isoprene-emitting and nonemitting plants. Thylakoid membrane stiffness was also measured using atomic force microscope (AFM) to identify a possible mode of action of isoprene in improving photochemical efficiency and photosynthetic stability. - We show that, when compared with nonemitters, isoprene-emitting tobacco plants exposed at high temperatures display a reduced increase of the NPQ energy-dependent component (qE) and stable (1) chlorophyll fluorescence lifetime; (2) amplitude of the fluorescence decay components; and (3) thylakoid membrane stiffness. - Our study shows for the first time that isoprene maintains PSII stability at high temperatures by preventing the modifications of the surrounding environment, namely providing a more steady and homogeneous distribution of the light-absorbing centres and a stable thylakoid membrane stiffness. Isoprene photoprotects leaves with a mechanism alternative to NPQ, enabling plants to maintain a high photosynthetic rate at rising temperatures.
Ajuts:	European Commission 610028 Agencia Estatal de Investigación DPI2017-83721-P
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Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	(High) temperature ; Atomic force microscopy (AFM) ; Chlorophyll fluorescence (quenching and lifetime) ; Fluorescence lifetime imaging microscopy (FLIM) ; Isoprene ; Nonphotochemical quenching (NPQ) ; Photosynthesis
Publicat a:	The new phytologist, Vol. 223, issue 3 (Aug. 2019) , p. 1307-1318, ISSN 1469-8137

DOI: 10.1111/nph.15847
PMID: 30980545

Postprint 37 p, 448.3 KB

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