Osteogenic commitment of Wharton's jelly mesenchymal stromal cells : Mechanisms and implications for bioprocess development and clinical application
Cabrera-Pérez, Raquel (Hospital Universitari Vall d'Hebron)
Monguió-Tortajada, Marta (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gámez-Valero, Ana (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Rojas-Márquez, R. (Centre de Regulació Genòmica)
Borràs i Serres, Francesc Enric (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Rudilla, F. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Vives Armengol, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina)

Date:	2019
Abstract:	Background: Orthopaedic diseases are one of the major targets for regenerative medicine. In this context, Wharton's jelly (WJ) is an alternative source to bone marrow (BM) for allogeneic transplantation since its isolation does not require an invasive procedure for cell collection and does not raise major ethical concerns. However, the osteogenic capacity of human WJ-derived multipotent mesenchymal stromal cells (MSC) remains unclear. Methods: Here, we compared the baseline osteogenic potential of MSC from WJ and BM cell sources by cytological staining, quantitative real-time PCR and proteomic analysis, and assessed chemical and biological strategies for priming undifferentiated WJ-MSC. Concretely, different inhibitors/activators of the TGFβ1-BMP2 signalling pathway as well as the secretome of differentiating BM-MSC were tested. Results: Cytochemical staining as well as gene expression and proteomic analysis revealed that osteogenic commitment was poor in WJ-MSC. However, stimulation of the BMP2 pathway with BMP2 plus tanshinone IIA and the addition of extracellular vesicles or protein-enriched preparations from differentiating BM-MSC enhanced WJ-MSC osteogenesis. Furthermore, greater outcome was obtained with the use of conditioned media from differentiating BM-MSC. Conclusions: Altogether, our results point to the use of master banks of WJ-MSC as a valuable alternative to BM-MSC for orthopaedic conditions.
Grants:	Instituto de Salud Carlos III RD16-0011-0028 Instituto de Salud Carlos III RD16-00111-0006 Instituto de Salud Carlos III PI19-01788 Instituto de Salud Carlos III RD16-0009 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR719 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR301 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR483
Note:	Altres ajuts: Fundació la Marató de TV3 (expedient number:122831).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bone marrow ; Bone regeneration ; Mesenchymal stromal cells ; Osteogenic differentiation ; Wharton's jelly
Published in:	Stem cell research & therapy, Vol. 10 Núm. 1 (28 2019) , p. 356, ISSN 1757-6512

DOI: 10.1186/s13287-019-1450-3
PMID: 31779673

11 p, 7.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles