Web of Science: 10 cites, Scopus: 11 cites, Google Scholar: cites
A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates
Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries)
Cano-Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Fàbregas, F. (Institut de Recerca i Tecnologia Agroalimentàries)
Roca-Pinilla, R. (Institut de Recerca i Tecnologia Agroalimentàries)
Seras-Franzoso, Joaquín (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Bach, Àlex (Institució Catalana de Recerca i Estudis Avançats)
Devant, M. (Institut de Recerca i Tecnologia Agroalimentàries)
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries)
García Fruitós, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data: 2018
Resum: The production of pure and soluble proteins is a complex, protein-dependent and time-consuming process, in particular for those prone-to-aggregate and/or difficult-to-purify. Although Escherichia coli is widely used for protein production, recombinant products must be co-purified through costly processes to remove lipopolysaccharide (LPS) and minimize adverse effects in the target organism. Interestingly, Lactococcus lactis, which does not contain LPS, could be a promising alternative for the production of relevant proteins. However, to date, there is no universal strategy to produce and purify any recombinant protein, being still a protein-specific process. In this context and considering that L. lactis is also able to form functional protein aggregates under overproduction conditions, we explored the use of these aggregates as an alternative source of soluble proteins. In this study, we developed a widely applicable and economically affordable protocol to extract functional proteins from these nanoclusters. For that, two model proteins were used: mammary serum amyloid A3 (M-SAA3) and metalloproteinase 9 (MMP-9), a difficult-to-purify and a prone-to-aggregate protein, respectively. The results show that it is possible to obtain highly pure, soluble, LPS-free and active recombinant proteins from L. lactis aggregates through a cost-effective and simple protocol with special relevance for difficult-to-purify or highly aggregated proteins.
Nota: Número d'acord de subvenció MINECO/RTA2015-00064-C02-01
Nota: Número d'acord de subvenció MINECO/RTA2015-00064-C02-02
Drets: This is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: Bacterial Proteins ; Cost-Benefit Analysis ; Lactococcus lactis ; Protein Aggregates ; Recombinant Proteins
Publicat a: Scientific reports (Nature Publishing Group), Vol. 8 (2018) , art. 13917, ISSN 2045-2322

DOI: 10.1038/s41598-018-32213-8
PMID: 30224788


10 p, 1.5 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut de Biotecnologia i de Biomedicina (IBB)
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 Registre creat el 2020-06-22, darrera modificació el 2021-03-02



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