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Página principal > Artículos > Artículos publicados > Assembly of histidine-rich protein materials controlled through divalent cations |
Fecha: | 2019 |
Resumen: | Nanostructured protein materials show exciting biomedical applications, since both structure and function can be genetically programmed. In particular, self-assembling histidine-rich proteins benefit from functional plasticity that allows the generation of protein-only nanoparticles for cell targeted drug delivery. However, the rational development of constructs with improved functions is limited by a poor control of the oligomerization process. By exploring cross-interactions between histidine-tagged building blocks, we have identified a critical architectonic role of divalent cations. The obtained data instruct about how histidine-rich protein materials can be assembled, disassembled and reassembled within the nanoscale through the stoichiometric manipulation of divalent ions, in a biochemical approach to biomaterials design. Statement of Significance: Divalent metal and non-metal cations such as Ni, Cu Ca and Zn have been identified as unexpected molecular tools to control the assembling, disassembling and reassembling of histidine-rich protein materials at the nanoscale. Their stoichiometric manipulation allows generating defined protein-protein cross-molecular contacts between building blocks, for a powerful nano-biochemical manipulation of the material's architecture. |
Ayudas: | Ministerio de Ciencia e Innovación BIO2016-76063-R Instituto de Salud Carlos III PI15/00272 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/FI_B100063 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | Protein materials ; Functional materials ; Nanoparticles ; Genetic design ; Controlled oligomerization |
Publicado en: | Acta Biomaterialia, Vol. 83 (Jan. 2019) , p. 257-264, ISSN 1878-7568 |
Article. Postprint 16 p, 406.6 KB |
Figura 1 1 p, 320.4 KB |
Figura 2 1 p, 146.8 KB |
Figura 3 1 p, 112.7 KB |
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Figura 5 1 p, 351.8 KB |
Llegendes de les figures 2 p, 147.5 KB |
Imatge 1 p, 517.0 KB |