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Pàgina inicial > Articles > Articles publicats > Engineering protein nanoparticles out from components of the human microbiome |
Data: | 2020 |
Resum: | Nanoscale protein materials are highly convenient as vehicles for targeted drug delivery because of their structural and functional versatility. Selective binding to specific cell surface receptors and penetration into target cells require the use of targeting peptides. Such homing stretches should be incorporated to larger proteins that do not interact with body components, to prevent undesired drug release into nontarget organs. Because of their low interactivity with human body components and their tolerated immunogenicity, proteins derived from the human microbiome are appealing and fully biocompatible building blocks for the biofabrication of nonreactive, inert protein materials within the nanoscale. Several phage and phage-like bacterial proteins with natural structural roles are produced in Escherichia coli as polyhistidine-tagged recombinant proteins, looking for their organization as discrete, nanoscale particulate materials. While all of them self-assemble in a variety of sizes, the stability of the resulting constructs at 37 °C is found to be severely compromised. However, the fine adjustment of temperature and Zn concentration allows the formation of robust nanomaterials, fully stable in complex media and under physiological conditions. Then, microbiome-derived proteins show promise for the regulatable construction of scaffold protein nanomaterials, which can be tailored and strengthened by simple physicochemical approaches. |
Ajuts: | Ministerio de Ciencia e Innovación BIO2016-76063-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Instituto de Salud Carlos III CP19/00028 Agència de Gestió d'Ajuts Universitaris i de Recerca 2018FI_B2_00051 Agència de Gestió d'Ajuts Universitaris i de Recerca 2019FI_B00352 Ministerio de Ciencia e Innovación FPU18/04615 |
Nota: | Altres ajuts: ICREA ACADEMIA award |
Drets: | Tots els drets reservats. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Microbiome ; Nanoparticles ; Protein engineering ; Protein materials ; Self-assembling protein |
Publicat a: | Small, Vol. 16, Issue 30 (July 2020) , art. 2001885, ISSN 1613-6829 |
Postprint 26 p, 681.2 KB |
Fig. 1 1 p, 264.7 KB |
Fig. 2 1 p, 193.8 KB |
Fig. 3 1 p, 604.5 KB |
Graphical abstract 1 p, 49.8 KB |
Supp. 1 1 p, 27.8 KB |
Supp. 2 1 p, 242.3 KB |